Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator

被引：5

作者：

Matlashov, Mikhail E. ^{[1
,2
]}

Vera, Jorge ^{[3
]}

Kasatkina, Ludmila A. ^{[1
,2
]}

Khodakhah, Kamran ^{[3
]}

Verkhusha, Vladislav V. ^{[1
,2
,4
]}

机构：

[1] Albert Einstein Coll Med, Dept Genet, Bronx, NY 10461 USA

[2] Albert Einstein Coll Med, Gruss Lipper Biophoton Ctr, Bronx, NY 10461 USA

[3] Albert Einstein Coll Med, Dept Neurosci, Bronx, NY USA

[4] Univ Helsinki, Fac Med, Medicum, Helsinki, Finland

来源：

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2022年 / 10卷

关键词：

GECI; iRFP; FRET; calcium imaging; far-red; biosensor; PROTEINS; NEURONS;

D O I：

10.3389/fcell.2022.880107

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are becoming powerful tools for neuroscience. Because of their spectral characteristics, the use of NIR GECIs helps to avoid signal loss from the absorption by body pigments, light-scattering, and autofluorescence in mammalian tissues. In addition, NIR GECIs do not suffer from cross-excitation artifacts when used with common fluorescent indicators and optogenetics actuators. Although several NIR GECIs have been developed, there is no NIR GECI currently available that would combine the high brightness in cells and photostability with small size and fast response kinetics. Here, we report a small FRET-based NIR fluorescent calcium indicator iGECInano. We characterize iGECInano in vitro, in non-neuronal mammalian cells, and primary mouse neurons. iGECInano demonstrates the improvement in the signal-to-noise ratio and response kinetics compared to other NIR GECIs.

引用

页数：11

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