Jiangtang decoction ameliorate diabetic nephropathy through the regulation of PI3K/Akt-mediated NF-κB pathways in KK-Ay mice

被引:53
作者
Hong, Jin-Ni [1 ]
Li, Wei-Wei [1 ]
Wang, Lin-Lin [1 ]
Guo, Hao [2 ]
Jiang, Yong [3 ]
Gao, Yun-Jia [3 ]
Tu, Peng-Fei [3 ]
Wang, Xue-Mei [1 ]
机构
[1] Peking Univ, Hosp 1, Integrated Lab Tradit Chinese Med & Western Med, Beijing, Peoples R China
[2] China Acad Chinese Med Sci, Xiyuan Hosp, Inst Basic Med Sci, Beijing, Peoples R China
[3] Peking Univ, Sch Pharmaceut Sci, Beijing, Peoples R China
来源
CHINESE MEDICINE | 2017年 / 12卷
基金
中国国家自然科学基金;
关键词
Diabetic nephropathy; Jiangtang decoction; Inflammation; Traditional Chinese Medicine; EUPHORBIA-HUMIFUSA WILLD; GLYCATION END-PRODUCTS; DB/DB MICE; PHOSPHOINOSITIDE; 3-KINASE; INFLAMMATORY DISEASE; TNF-ALPHA; PATHOGENESIS; POLYSACCHARIDES; INHIBITION; MECHANISMS;
D O I
10.1186/s13020-017-0134-0
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Background: Jiangtang decoction (JTD) is a China patented drug which contains Euphorbia humifusa Willd, Salvia miltiorrhiza Bunge, Astragalus mongholicus Bunge, Anemarrhena asphodeloides Bunge, and Coptis chinensis Franch. For decades, it has also been used clinically to treat diabetic nephropathy (DN) effectively; however, the associated mechanisms remain unknown. Thus, the present study aimed to examine the protective efficacy of JTD in DN and elucidate the underlying molecular mechanisms. Methods: A diabetic model using KK-Ay mice received a daily administration of JTD for 12 weeks. Body weight, blood glucose, triglycerides (TGs), total cholesterol (TC), urea nitrogen (UN), creatinine (Cr), and microalbumin/urine creatinine (MA/UCREA) was measured every 4 weeks. Furthermore, on the day of the sacrifice, blood, urine, and kidneys were collected to assess renal function according to general parameters. Pathological staining was performed to evaluate the protective renal effect of JTD. In addition, the levels of inflammatory cytokines (tumor necrosis factor-a [TNF-alpha], interleukin [IL]-6 and intercellular adhesion molecule [ICAM]-1), insulin receptor substrate [IRS]-1, advanced glycation end products [AGEs], and receptor of glycation end products [RAGE] were assessed. Finally, the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway and involvement of nuclear factor-kappa B (NF-kappa B) was further analyzed. Results: After 12 weeks of metformin and JTD administration, the mice exhibited a significant amelioration in glucose and lipid metabolism dysfunction, reduced morphological changes in the renal tissue, decreased urinary albumin excretion, and normalized creatinine clearance. JTD treatment also reduced the accumulation of AGEs and RAGE, up-regulated IRS-1, and increased the phosphorylation of both PI3K (p85) and Akt, indicating that the activation of the PI3K/Akt signaling pathway was involved. Additionally, JTD administration reduced the elevated levels of renal inflammatory mediators and decreased the phosphorylation of NF-kappa B p65. Conclusions: These results demonstrate that JTD might reduce inflammation in DN through the PI3K/Akt and NF-kappa B signaling pathways.
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页数:16
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