Usnic acid ameliorates bleomycin-induced pulmonary fibrosis in mice via inhibition of inflammatory responses and oxidative stress

被引：5
作者：
Huang, Xiao-Qi [1 ,2 ]
Ai, Gao-Xiang [1 ]
Zheng, Xing-Han [1 ]
Liao, Hui-Jun [3 ]
机构：
[1] Guangzhou Univ Chinese Med, Math Engn Acad Chinese Med, Guangdong Prov Key Lab New Drug Dev & Res Chinese, Guangzhou 510006, Guangdong, Peoples R China
[2] Guangzhou Univ Chinese Med, Dongguan Math Engn Acad Chinese Med, Dongguan 523808, Peoples R China
[3] Shenzhen Sixth Peoples Hosp, Nanshan Hosp, Dept Clin Pharm & Pharmaceut Serv, Shenzhen, Guangdong, Peoples R China
来源：
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH | 2019年 / 18卷 / 12期
关键词：
Usnic acid; Bleomycin; Pulmonary fibrosis; Inflammation; Oxidative stress; ACUTE LUNG INJURY; DISEASE SEVERITY; TRANSPLANTATION; MARKER;
D O I：
10.4314/tjpr.v18i12.15
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Purpose: To Investigate the effect of usnic acid (UA) on bleomycin (BLM)-induced pulmonary fibrosis in mice, and the underlying mechanism. Methods: Male Kunming mice with bleomycin-induced pulmonary fibrosis (PF) were exposed to different concentrations of usnic acid. Lung coefficient and histopathological changes were determined, while MDA, superoxide dismutase (SOD) activity, and expression levels of hydroxyproline, tumor necrosis factor-alpha, interleukins-1 beta & 6, and transforming growth factor-beta 1 were assayed in lung homogenates. Results: UA significantly mitigated lung coefficient and histopathological changes in mice. Compared to the bleomycin group, MDA level was significantly reduced while the content of SOD markedly increased after UA pretreatment (p < 0.05). Moreover, UA significantly reduced the expression levels of all the parameters, relative to bleomycin group (p < 0.05). Conclusion: These results indicate that UA protects mice against bleomycin-induced PF via a mechanism associated with attenuation of pro-oxidant stress and inflammation. Therefore, UA has therapeutic potential for the management of pulmonary fibrosis.
引用
收藏
页码：2563 / 2569
页数：7
相关论文
共 23 条
[1]   Effect of Resveratrol on Treatment of Bleomycin-Induced Pulmonary Fibrosis in Rats [J].
Akgedik, Recep ;
Akgedik, Sukran ;
Karamanli, Harun ;
Uysal, Sema ;
Bozkurt, Bulent ;
Ozol, Duygu ;
Armutcu, Ferah ;
Yildirim, Zeki .
INFLAMMATION, 2012, 35 (05) :1732-1741
[2]   Respirable dry powder formulation of bleomycin for developing a pulmonary fibrosis animal model [J].
Aoki, Yosuke ;
Kojo, Yoshiki ;
Yamada, Shizuo ;
Onoue, Satomi .
JOURNAL OF PHARMACEUTICAL SCIENCES, 2012, 101 (06) :2074-2081
[3]   Epithelial repair mechanisms in the lung [J].
Crosby, Lynn M. ;
Waters, Christopher M. .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2010, 298 (06) :L715-L731
[4]   Serum levels of oxidative stress as a marker of disease severity in idiopathic pulmonary fibrosis [J].
Daniil, Zoe D. ;
Papageorgiou, Evangelia ;
Koutsokera, Agela ;
Kostikas, Konstantinos ;
Kiropoulos, Theodoros ;
Papaioannou, Andriana I. ;
Gourgoulianis, Konstantinos I. .
PULMONARY PHARMACOLOGY & THERAPEUTICS, 2008, 21 (01) :26-31
[5]  
Freeburn RW, 2001, EUR CYTOKINE NETW, V12, P33
[6]   Integrative analyses of miRNA and proteomics identify potential biological pathways associated with onset of pulmonary fibrosis in the bleomycin rat model [J].
Fukunaga, Satoki ;
Kakehashi, Anna ;
Sumida, Kayo ;
Kushida, Masahiko ;
Asano, Hiroyuki ;
Gi, Min ;
Wanibuchi, Hideki .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2015, 286 (03) :188-197
[7]   Current and emerging drugs for idiopathic pulmonary fibrosis [J].
Gharaee-Kermani, Mehrnaz ;
Hu, Biao ;
Thannickal, Victor J. ;
Phan, Sern. H. ;
Gyetko, Margaret R. .
EXPERT OPINION ON EMERGING DRUGS, 2007, 12 (04) :627-646
[8]   Usnic acid [J].
Ingólfsdóttir, K .
PHYTOCHEMISTRY, 2002, 61 (07) :729-736
[9]   Oxidative stress, plasminogen activator inhibitor 1, and lung fibrosis [J].
Liu, Rui-Ming .
ANTIOXIDANTS & REDOX SIGNALING, 2008, 10 (02) :303-319
[10]   Oxy radicals, lipid peroxidation and DNA damage [J].
Marnett, LJ .
TOXICOLOGY, 2002, 181 :219-222
123