The effect of pre-analytical handling on the stability of fractalkine, monocyte chemoattractant protein 1 (MCP1), interleukin 6 and interleukin 8 in samples of human cerebrospinal fluid

被引：0

作者：

Mortensen, Winnie Charlotte Pedersen ^{[1
]}

Bendix, Laila ^{[2
]}

Jensen, Hanne Irene ^{[1
,3
]}

Varnum, Claus ^{[3
,4
]}

Rasmussen, Lasse Enkebolle ^{[4
]}

Lauridsen, Jorgen T. ^{[5
]}

Borbye-Lorenzen, Nis ^{[6
]}

Skogstrand, Kristin ^{[6
]}

Toft, Palle ^{[7
,8
]}

Vaegter, Henrik Bjarke ^{[2
,9
]}

Blichfeldt-Eckhardt, Morten Rune ^{[2
,9
]}

机构：

[1] Univ Hosp Southern Denmark, Lillebaelt Hosp, Dept Anesthesia, Beriderbakken 4, DK-7100 Vejle, Denmark

[2] Odense Univ Hosp, Pain Ctr, Pain Res Grp, Heden 9, DK-5000 Odense, Denmark

[3] Univ Southern Denmark, Dept Reg Hlth Res, Winslowpk 19, DK-5000 Odense, Denmark

[4] Univ Hosp Southern Denmark, Lillebaelt Hosp, Dept Orthoped Surg, Beriderbakken 4, DK-7100 Vejle, Denmark

[5] Univ Southern Denmark, Dept Business & Econ, Campusvej 55, DK-5230 Odense, Denmark

[6] Statens Serum Inst SSI, Danish Ctr Neonatal Screening, Dept Congenital Disorders, Artillerivej 5, DK-2300 Copenhagen, Denmark

[7] Odense Univ Hosp, Dept Anesthesiol, JB Winslows Vej 4, DK-5000 Odense, Denmark

[8] Odense Univ Hosp, Intens Care Unit, JB Winslows Vej 4, DK-5000 Odense, Denmark

[9] Univ Southern Denmark, Fac Hlth Sci, Dept Clin Res, Winslowpk 19, DK-5000 Odense, Denmark

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2021年 / 494卷

关键词：

Pre-analytical handling; Time delay; Storage temperature; CX3CL1 (C-X3-C motif chemokine ligand 1); CCL-2 (C-C motif chemokine ligand 2); CHRONIC PAIN; STANDARDIZATION; OPTIMIZATION; CSF;

D O I：

10.1016/j.jim.2021.113057

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytokine networks in cerebrospinal fluid (CSF) are important to our understanding of several neuroinflammatory diseases. Knowledge about optimal handling of samples is limited but important to minimize bias and reduce costs in CSF biomarker studies. The aim of this study was to examine the effect of storage temperature and time delay from CSF sample collection until freezing on the concentration of 11 different cytokines thought to be associated with chronic pain. CSF samples from 21 individuals undergoing hip or knee arthroplasty under spinal anesthesia were divided between two tubes. One tube was stored and centrifuged (within 30 min) at room temperature, and one tube was stored in ice water and centrifuged (within 30 min) at 4 degrees C. Each tube was split into six vials that were frozen at -80 degrees C, 0.5, 1, 2, 3, 4, or 5 h after collection. Cytokines were analyzed using a multiplex panel. A random effect panel data regression was conducted for each biomarker including the variables of storage temperature until freezing and time delay. Four cytokines had detectable levels: Fractalkine, monocyte chemoattractant protein 1 (MCP-1), interleukine 6 (IL-6), and interleukine 8 (IL-8). There was no significant effect of storage temperature and time delay on MCP-1, IL-6, or IL-8 concentrations. Fractalkine concentration showed no clear trend. No concentration differences were observed between samples kept in ice water and those at mom temperature except at the 3-h time point, and there was no overall significant effect of time delay on fractalkine concentration. We found no clear effect of storage temperature and time delay up to five hours from sample collection until freezing on the CSF concentrations of fractalkine, MCP-1, IL-6, or IL-8.

引用

页数：5