Multiple asters organize the yolk microtubule network during dclk2-GFP zebrafish epiboly

被引:0
作者
Marsal, Maria [1 ]
Bernardello, Matteo [1 ]
Gualda, Emilio J. [1 ]
Loza-Alvarez, Pablo [1 ]
机构
[1] Barcelona Inst Sci & Technol, ICFO Inst Ciencies Foton, Barcelona, Spain
来源
SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期
基金
欧盟地平线“2020”;
关键词
SELF-ORGANIZATION; DYNAMIC MICROTUBULES; SYNCYTIAL LAYER; CELL BIOLOGY; IN-VIVO; NUCLEATION; MICROSCOPY; PLATFORM; LIVE; CENTROSOMES;
D O I
10.1038/s41598-022-07747-7
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
It is known that the organization of microtubule (MT) networks in cells is orchestrated by subcellular structures named MT organizing centers (MTOCs). In this work, we use Light Sheet Fluorescence and Confocal Microscopy to investigate how the MT network surrounding the spherical yolk is arranged in the dclk2-GFP zebrafish transgenic line. We found that during epiboly the MT network is organized by multiple aster-like MTOCS. These structures form rings around the yolk sphere. Importantly, in wt embryos, aster-like MTOCs are only found upon pharmacological or genetic induction. Using our microscopy approach, we underscore the variability in the number of such asters in the transgenic line and report on the variety of global configurations of the yolk MT network. The asters' morphology, dynamics, and their distribution in the yolk sphere are also analyzed. We propose that these features are tightly linked to epiboly timing and geometry. Key molecules are identified which support this asters role as MTOCs, where MT nucleation and growth take place. We conclude that the yolk MT network of dclk2-GFP transgenic embryos can be used as a model to organize microtubules in a spherical geometry by means of multiple MTOCs.
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页数:15
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