Detection of BRAF V600E Mutations With Next-Generation Sequencing in Infarcted Thyroid Carcinomas After Fine-Needle Aspiration

被引:0
|
作者
Kouba, Erik [1 ]
Ford, Andrew [2 ]
Brown, Charmaine G. [2 ]
Yeh, Chen [2 ]
Siegal, Gene P. [1 ]
Manne, Upender [1 ]
Eltoum, Isam-Eldin [1 ]
机构
[1] Univ Alabama Birmingham, Dept Pathol, 508 20th St South HSB 149M, Birmingham, AL 35249 USA
[2] Circulogene, Birmingham, AL USA
关键词
BRAF V600E; Thyroid carcinoma; Next-generation sequencing; Fine-needle aspiration; Fine-needle aspiration infarction; BRAF(V600E) MUTATION; PHOSPHATIDYLINOSITOL; 3-KINASE/AKT; HISTOLOGIC ALTERATIONS; BETHESDA SYSTEM; ADULT PATIENTS; CANCER; NODULES; MANAGEMENT; DIAGNOSIS; BIOPSY;
D O I
10.1093/AJCP/AQY045
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Objectives: Fine-needle aspiration (FNA) of thyroid lesions may result in infarction and diagnostic difficulties on subsequent thyroidectomy specimens. Next-generation sequencing (NGS) methods for detection of hallmark driver BRAF V600E mutations may help characterize such tumors in which histologic alterations preclude definitive tissue diagnosis. Methods: Thyroidectomy specimens with both malignant FNA diagnoses and resultant infarction were identified from our institutional database. NGS methods were used to detect BRAF V600E mutations in the infarcted thyroid carcinomas. Results: Nine thyroid carcinomas with infarction were characterized as BRAF-like papillary thyroid carcinoma based on molecular driver categorization and histologic diagnosis. BRAF V600E mutations were detected in the infarcted tissue in four (67%) of six lesions. Conclusions: We demonstrate detection of hallmark BRAF V600E mutations by NGS within infarcted tissue of thyroid carcinomas after FNA. This suggests a potential ancillary method of characterizing infarcted thyroid carcinomas whose altered histology may be nondiagnostic.
引用
收藏
页码:177 / 185
页数:9
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