GLAST:: gene expression regulation by phorbol esters

被引：24

作者：

Espinoza-Rojo, M ^{[1
]}

López-Bayghen, E ^{[1
]}

Ortega, A ^{[1
]}

机构：

[1] Inst Politecn Nacl, CINVESTAV, Dept Genet & Mol Biol, Mexico City 07000, DF, Mexico

来源：

NEUROREPORT | 2000年 / 11卷 / 12期

关键词：

L-aspartate; Bergmann glia; GLAST; L-glutamate;

D O I：

10.1097/00001756-200008210-00043

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The gene expression regulation of the Na+-dependent high affinity glutamate/aspartate transporter GLAST expressed in cultured Bergmann glia cells from chick cerebellum was studied. A 679bp fragment of the chick GLAST cDNA was cloned and sequenced. Specific PCR primers were used to quantify chick GLAST mRNA levels. Treatment of the cells with the Ca2+/diacylglycerol dependent protein kinase C (PKC) activator, phorbol 12-tetradecanoyl-13-acetate (TPA) produced a decrease in transporter mRNA levels, without an effect in its mRNA half life, suggesting a transcriptional down regulation. Activation of the cAMP pathway results in a transient decrease in GLAST mRNA levels, in contrast with the TPA effect. These findings suggest that GLAST expression is under control of distinct signaling pathways. (C) 2000 Lippincott Williams & Wilkins.

引用

页码：2827 / 2832

页数：6

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