Role of myosin regulatory light chain and Rac1 in the migration of polyamine-depleted intestinal epithelial cells

We have previously shown that polyamine depletion decreased migration, Rac activation, and protein serine threonine phosphatase 2A activity. We have also shown that polyamine depletion increased cortical F- actin and decreased lamellipodia and stress fibers. In this study, we used staurosporine ( STS), a potent, cell- permeable, and broad- spectrum serine/ threonine kinase inhibitor, and studied migration. STS concentrations above 100 nM induced apoptosis. However, in polyamine- depleted cells, a lower concentration of STS ( 5 nM) increased attachment, spreading, Rac1 activation, and, subsequently, migration without causing apoptosis. STS- induced migration was completely prevented by a Rac1 inhibitor ( NSC- 23766) and dominant negative Rac1. These results imply that STS restores migration in polyamine- depleted cells through Rac1. The most important finding in this study was that polyamine depletion increased the association of phosphorylated myosin regulatory light chain ( pThr(18)/ Ser(19)- MRLC) at the cell periphery, which colocalized with thick cortical F- actin. Localization of pThr(18)-and pSer(19)- MRLC was found with stress fibers and nuclei, respectively. STS decreased the phosphorylation of cellular and peripheral pThr(18)- MRLC without any effect on nuclear pSer19- MRLC, dissolved thick cortical F- actin, and increased lamellipodia and stress fiber formation in polyamine- depleted cells. In control and polyaminedepleted cells, focal adhesion kinase ( FAK) colocalized with stress fibers and the actin cortex, respectively. STS reorganized FAK, paxillin, and the cytoskeleton. These results suggest that polyamine depletion prevents the dephosphorylation of MRLC and thereby prevents the dynamic reorganization of the actin cytoskeleton and decreases lamellipodia formation resulting in the inhibition of migration.