Protein profile analysis by SDS-PAGE of Mycoplasma gallisepticum strains S6(208) and F-K810 grown in Hayflick's and Frey's media

被引:0
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作者
Ferraz, PN
Menezes, CCP
Danelli, MDM
Lizeu, JDP
do Nascimento, ER
Lucchesi, DM
机构
[1] Univ Fed Rural Rio De Janeiro, Inst Vet, Dept Microbiol & Imunol, BR-23890000 Seropedica, RJ, Brazil
[2] Univ Fed Fluminense, Fac Vet, Niteroi, RJ, Brazil
[3] Fiocruz MS, Inst Tecnol Imunobiol, BR-21045900 Rio De Janeiro, Brazil
关键词
Mycoplasma gallisepticum; SDS-PAGE; protein profile;
D O I
暂无
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Mycoplasma gallisepticum strains [wild-type S6 (208) and a vaccine-type F-EC810] grown in Frey's and Hayflick's media were analyzed by SDS-PAGE. No visual changes in the protein profiles of these strains were observed regardless of the media composition used, although the polyacrylamide gel electrophoretograms showed minor differences do exist when densitometer traces of the gel are compared. Both strains were easily differentiated on SDS-PAGE analysis by a peptide band p75, that is specific for MG F-K810 strain, used as vaccine.
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页码:113 / 115
页数:3
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