Generation of CsI Cluster Ions for Mass Calibration in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

被引：43

作者：

Lou, Xianwen ^{[1
]}

van Dongen, Joost L. J. ^{[1
]}

Meijer, E. W. ^{[1
]}

机构：

[1] Eindhoven Univ Technol, Lab Macromol & Organ Chem, NL-5600 MB Eindhoven, Netherlands

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2010年 / 21卷 / 07期

关键词：

TIME-OF-FLIGHT; DESORPTION IONIZATION; ACCURATE MASS; DELAYED EXTRACTION; HIGH-RESOLUTION; ELECTROSPRAY-IONIZATION; MALDI; SPECTRA; PROTEINS; VELOCITY;

D O I：

10.1016/j.jasms.2010.02.029

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method was developed for the generation of cesium iodide (CsI) cluster ions up to m/z over 20,000 in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Calibration ions in both positive and negative ion modes can readily be generated from a single MALDI spot of CsI(3) with 2-[(2E)-3-(4-tert-butylphenyl)-2-methylprop-2-enylidene] malononitrile (DCTB) matrix. The major cluster ion series observed in the positive ion mode is [(CsI)(n)Cs](+), and in the negative ion mode is [(CsI)(n)I](-). In both cluster series, ions spread evenly every 259.81 units. The easy method described here for the production of CsI cluster ions should be useful for MALDI MS calibrations. (J Am Soc Mass Spectrom 2010, 21, 1223-1226) (C) 2010 American Society for Mass Spectrometry

引用

页码：1223 / 1226

页数：4

共 37 条

[1] QUALITATIVE ANALYSIS OF ORGANIC COMPOUNDS BY MASS SPECTROMETRY [J].

BEYNON, JH .

NATURE, 1954, 174 (4433) :735-737

[2] Intercomparison study on accurate mass measurement of small molecules in mass spectrometry [J].

Bristow, AWT ;

Webb, KS .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2003, 14 (10) :1086-1098

[3] Accurate mass measurement for the determination of elemental formula - A tutorial [J].

Bristow, AWT .

MASS SPECTROMETRY REVIEWS, 2006, 25 (01) :99-111

[4] Improved calibration of time-of-flight mass spectra by simplex optimization of electrostatic ion calculations [J].

Christian, NP ;

Arnold, RJ ;

Reilly, JP .

ANALYTICAL CHEMISTRY, 2000, 72 (14) :3327-3337

[5] Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass measurement accuracy by using delayed extraction [J].

Edmondson, RD ;

Russell, DH .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1996, 7 (10) :995-1001

[6]

Glückmann M, 1999, J MASS SPECTROM, V34, P467, DOI 10.1002/(SICI)1096-9888(199905)34:5<467::AID-JMS809>3.0.CO

[7]

2-8

[8] A calibration method that simplifies and improves accurate determination of peptide molecular masses by MALDI-TOF MS [J].

Gobom, J ;

Mueller, M ;

Egelhofer, V ;

Theiss, D ;

Lehrach, H ;

Nordhoff, E .

ANALYTICAL CHEMISTRY, 2002, 74 (15) :3915-3923

[9] A simple algorithm improves mass accuracy to 50-100 ppm for delayed extraction linear matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J].

Hack, CA ;

Benner, WH .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (13) :1304-1312

[10]

Hop CECA, 1996, J MASS SPECTROM, V31, P1314, DOI 10.1002/(SICI)1096-9888(199611)31:11<1314::AID-JMS429>3.0.CO

← 1 2 3 4 →