A rapid, sensitive, and widely applicable method for quantitative analysis of underivatized amino acids in different biological matrices by UHPLC-MS/MS

被引：13

作者：

Guo, Na ^{[1
]}

Yang, Dawei ^{[2
]}

Yang, Xiaofang ^{[1
]}

Yan, Han ^{[1
]}

Fan, Bin ^{[1
]}

Dai, Jingang ^{[1
]}

Lei, Yan ^{[1
]}

Yan, Dan ^{[1
,3
]}

机构：

[1] China Acad Chinese Med Sci, Expt Res Ctr, Beijing 100700, Peoples R China

[2] Liaocheng Peoples Hosp, Zhong Yuan Acad Biol Med, Liaocheng, Shandong, Peoples R China

[3] Capital Med Univ, Beijing Shijitan Hosp, Beijing, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2019年 / 42卷 / 20期

基金：

中国国家自然科学基金;

关键词：

amino acids; biological matrices; tandem mass spectrometry; ultra high performance liquid chromatography; TANDEM MASS-SPECTROMETRY; HUMAN PLASMA; CHROMATOGRAPHY; QUANTIFICATION; METABOLISM; VALIDATION; DIAGNOSIS;

D O I：

10.1002/jssc.201900299

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid, sensitive, and widely applicable method for the simultaneous quantitative analysis of 20 underivatized amino acids in different biological matrices, including serum, plasma, and tissue homogenates, using ultra high performance liquid chromatography with tandem mass spectrometry was developed and validated. Only 4 mu L of serum, plasma, or tissue homogenate was extracted with 996 mu L of solution (1.7 mM ammonium formate in 85% acetonitrile containing 0.1% formic acid) containing 100 ng/mL phenylalanine-d5 as an internal standard without any further derivatization step. In addition, the matrix effects were small because a large volume of extraction solution was used. The total run time including reequilibration was 13 min. The results of linearity, accuracy, repeatability, precision, limits of detection, limits of quantification, and sample stability were sufficient to allow the measurement of the amino acids in different biological matrices. We conclude that our method is rapid, sensitive, and widely applicable and represents an improvement over other currently available technologies.

引用

页码：3173 / 3181

页数：9

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