Tracking COVID-19 with wastewater to understand asymptomatic transmission

被引：29

作者：

Wannigama, Dhammika Leshan ^{[1
,2
,3
]}

Amarasiri, Mohan ^{[4
]}

Hurst, Cameron ^{[5
,6
]}

Phattharapornjaroen, Phatthranit ^{[7
,20
]}

Abe, Shuichi ^{[8
]}

Hongsing, Parichart ^{[9
,10
]}

Rad, S. M. Ali Hosseini ^{[11
,12
]}

Pearson, Lachlan ^{[13
]}

Saethang, Thammakorn ^{[14
]}

Luk-in, Sirirat ^{[15
]}

Kueakulpattana, Naris ^{[1
,2
]}

Storer, Robin James ^{[16
]}

Ounjai, Puey ^{[17
]}

Jacquet, Alain ^{[18
]}

Leelahavanichkul, Asada ^{[1
,19
]}

Chatsuwan, Tanittha ^{[1
,2
]}

机构：

[1] Chulalongkorn Univ, King Chulalongkorn Mem Hosp, Dept Microbiol, Thai Red Cross Soc,Fac Med, Bangkok, Thailand

[2] Chulalongkorn Univ, Antimicrobial Resistance & Stewardship Res Unit, Fac Med, Bangkok, Thailand

[3] Univ Western Australia, Sch Med, Fac Hlth & Med Sci, Nedlands, WA, Australia

[4] Kitasato Univ, Sch Allied Hlth Sci, Grad Sch Med Sci, Lab Environm Hyg,Dept Hlth Sci, Sagamihara, Kanagawa, Japan

[5] QIMR Berghofer Med Res Inst, Stat, Brisbane, Qld, Australia

[6] Thammasat Univ, Fac Publ Hlth, Khlong Luang Dist, Pathum Thani, Thailand

[7] Mahidol Univ, Ramathibodi Hosp, Dept Emergency Med, Ctr Excellence,Fac Med, Bangkok, Thailand

[8] Yamagata Prefectural Cent Hosp, Dept Infect Dis & Infect Control, Yamagata, Japan

[9] Mae Fah Luang Univ Hosp, Chiang Rai, Thailand

[10] Mae Fah Luang Univ, Sch Integrat Med, Chiang Rai, Thailand

[11] Univ Otago, Dept Microbiol & Immunol, Dunedin 9010, New Zealand

[12] Chulalongkorn Univ, Ctr Excellence Immunol & Immune Mediated Dis, Bangkok 10330, Thailand

[13] Westmead Inst Med Res, Ctr Heart Res, Sydney, NSW, Australia

[14] Kasetsart Univ, Dept Comp Sci, Fac Sci, Bangkok, Thailand

[15] Mahidol Univ, Fac Med Technol, Dept Clin Microbiol & Appl Technol, Bangkok, Thailand

[16] Chulalongkorn Univ, Off Res Affairs, Fac Med, Bangkok, Thailand

[17] Mahidol Univ, Dept Biol, Fac Sci, Bangkok, Thailand

[18] Chulalongkorn Univ, Chula Vaccine Res Ctr, Fac Med, Bangkok, Thailand

[19] Chulalongkorn Univ, Dept Microbiol, Translat Res Inflammat & Immunol Res Unit TRIRU, Bangkok, Thailand

[20] Gothenburg Univ, Sahlgrenska Acad, Inst Clin Sci, Dept Surg, S-40530 Gothenburg, Sweden

来源：

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES | 2021年 / 108卷

关键词：

COVID-19; Wastewater; Asymptomatic transmission; SARS-CoV-2 viral RNA; Wastewater of Bangkok; SARS-CoV-2 in wastewater;

D O I：

10.1016/j.ijid.2021.05.005

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Introduction: SARS-CoV-2 RNA is excreted in feces of most patients, therefore viral load in wastewater can be used as a surveillance tool to develop an early warning system to help and manage future pandemics. Methods: We collected wastewater from 24 random locations at Bangkok city center and 26 nearby suburbs from July to December 2020. SARS-CoV-2 RNA copy numbers were measured using real-time polymerase chain reaction (PCR). Results: SARS-CoV-2 RNA was detected in wastewater from both the city center and suburbs. Except for July, there were no significant differences in copy numbers between the city center and suburbs. Between October and November, a sharp rise in copy number was observed in both places followed by two to three times increase in December, related to SARS-CoV-2 cases reported for same month. Conclusions: Our study provided the first dataset related to SARS-CoV-2 viral RNA in the wastewater of Bangkok. Our results suggest that wastewater could be used as a complementary source for detecting viral RNA and predicting upcoming outbreaks and waves. (c) 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-ncnd/4.0/).

引用

页码：296 / 299

页数：4

共 11 条

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