Quantitative reverse transcription and polymerase chain reaction analysis of oxytocin and vasopressin receptor mRNAs in the rat uterus near parturition

被引:11
作者
Clerget, MS
Elalouf, JM
Germain, G
机构
[1] INRA, Ctr Rech Jouy En Josas, Lab Neurobiol Fonct Vegetat, F-78352 Jouy En Josas, France
[2] CEA Saclay, Serv Biol Cellulaire, Dept Biol Cellulaire & Mol, F-91191 Gif Sur Yvette, France
关键词
quantitative reverse transcription-polymerase chain reaction; oxytocin receptors; vasopressin receptors; rat uterus; gestation;
D O I
10.1016/S0303-7207(97)00218-9
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Oxytocin receptors (OT-R) are known to be involved in the course of labor since a massive increase in OT-binding sites is observed in the uterus just before parturition. Vasopressin (AVP)-binding sites have also been observed and have been shown to mediate uterotonic responses. To determine exactly which subtypes of OT/AVP receptors are expressed in the rat uterus near parturition, we carried out absolute quantitations of the neurohypophysial hormone receptor (OT-R and the vasopressin receptors V-1a-R, V-1b-R and V-2-R) mRNAs with an assay based on reverse transcription-polymerase chain reaction (RT-PCR) using in vitro transcribed mutated cRNAs as internal standards. The number of mRNA molecules/ng of total RNA was 35 +/- 6, 220 +/- 33 and 39 +/- 9 for OT-R (P < 0.01) and 16 +/- 1, 25 +/- 8 and 31 +/- 5 for V-1a-R (P > 0.05) on day (D) 21, 22 and 23 of gestation (post-parturient), respectively. We did not detect V-1b-R and V-2-R mRNAs in the pregnant uterus. Therefore, the heterogeneity of OT and AVP receptors in the rat uterus can only be assigned to the presence of OT-R and V-1a-R neurohypophysial hormone receptor subtypes, whereas V-1b-R and V-2-R can not be invoked. Only OT-R mRNA levels change in the uterus near parturition. (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:79 / 89
页数:11
相关论文
共 46 条
[1]   OXYTOCIN RECEPTORS AND PARTURITION .1. CONTROL OF OXYTOCIN RECEPTOR CONCENTRATION IN THE RAT MYOMETRIUM AT TERM [J].
ALEXANDROVA, M ;
SOLOFF, MS .
ENDOCRINOLOGY, 1980, 106 (03) :730-735
[2]  
ALEXANDROVA M, 1980, ENDOCRINOLOGY, V106, P736, DOI 10.1210/endo-106-3-736
[3]   Comparison of vasopressin and oxytocin receptors in the rat uterus and vascular tissue [J].
Anouar, A ;
MarieStephanie, C ;
Durroux, T ;
Barberis, C ;
Germain, G .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1996, 308 (01) :87-96
[4]  
Barberis C, 1996, CRIT REV NEUROBIOL, V10, P119
[5]  
BAUERDANTOIN AC, 1993, ENDOCRINOLOGY, V133, P1911, DOI 10.1210/en.133.4.1911
[6]   ABSOLUTE MESSENGER-RNA QUANTIFICATION USING THE POLYMERASE CHAIN-REACTION (PCR) - A NOVEL-APPROACH BY A PCR AIDED TRANSCRIPT TITRATION ASSAY (PATTY) [J].
BECKERANDRE, M ;
HAHLBROCK, K .
NUCLEIC ACIDS RESEARCH, 1989, 17 (22) :9437-9446
[7]   V(2)-LIKE VASOPRESSIN RECEPTOR MOBILIZES INTRACELLULAR CA2+ IN RAT MEDULLARY COLLECTING TUBULES [J].
CHAMPIGNEULLE, A ;
SIGA, E ;
VASSENT, G ;
IMBERTTEBOUL, M .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (01) :F35-F45
[8]   OXYTOCIN-BINDING AND VASOPRESSIN-BINDING SITES IN THE RAT UTERUS - COMPETITION BINDING AND INHIBITORY PA2 STUDIES WITH OXYTOCIN AND OXYTOCIN ANTAGONISTS [J].
CHAN, WY ;
CAO, L ;
HILL, PS ;
HRUBY, VJ .
ENDOCRINOLOGY, 1990, 126 (04) :2095-2101
[9]   OXYTOCIN RECEPTOR SUBTYPES IN THE PREGNANT RAT MYOMETRIUM AND DECIDUA - PHARMACOLOGICAL DIFFERENTIATIONS [J].
CHAN, WY ;
CHEN, DL ;
MANNING, M .
ENDOCRINOLOGY, 1993, 132 (03) :1381-1386
[10]  
CHEN DL, 1994, J REPROD FERTIL, V102, P337, DOI 10.1530/jrf.0.1020337