Separation of catechins and O-methylated (-)-epigallocatechin gallate using polyamide thin-layer chromatography

被引：12
|
作者：
Wang, Kunbo [1 ,2 ,3 ]
Chen, Qincao [3 ]
Lin, Yong [1 ,2 ,3 ]
Yu, Shuangshang [3 ]
Lin, Haiyan [1 ,2 ,3 ]
Huang, Jianan [1 ,2 ,3 ]
Liu, Zhonghua [1 ,2 ,3 ]
机构：
[1] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China
[2] Hunan Agr Univ, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China
[3] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2016年 / 1017卷
基金：
中国国家自然科学基金;
关键词：
Catechins; O-methylated (-)-epigallocatechin gallate; Thin-layer chromatography; Double-development; Tea; GREEN TEA CATECHINS; LIQUID-CHROMATOGRAPHY; PURINE ALKALOIDS; TLC SEPARATION; DERIVATIVES; CULTIVARS;
D O I：
10.1016/j.jchromb.2015.11.060
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Thin-layer chromatography (TLC) method for the separation and quantitative determination of seven related compounds: (+)-catechin (C), (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG), (-)-epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3 '' Me) and (-)-epigallocatechin- 3-O-(4-O-methyl) gallate (EGCG4 '' Me) has been developed. The above-mentioned seven compounds have been resolved using polyamide TLC plates using a double-development with methanol followed by acetone/acetic acid (2:1, v/v). In addition, separation of the phenolic acids namely gallic acid, chlorogenic acid, and caffeic acid was achieved using the same solvent system. The applicability of the method was checked by screening of extracts of green, black, oolong, white tea and tea cultivars leaves. (C) 2016 Elsevier B.V. All rights reserved.
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页码：221 / 225
页数：5
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