DNA polymerase-mediated DNA synthesis on a TNA template

被引：92
作者：
Chaput, JC
Ichida, JK
Szostak, JW [1 ]
机构：
[1] Massachusetts Gen Hosp, Howard Hughes Med Inst, Boston, MA 02114 USA
[2] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 04期
关键词：
D O I：
10.1021/ja028589k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
TNA, or threose nucleic acid, is capable of Watson-Crick base pairing with DNA, RNA, and TNA; coupled with its chemical simplicity, this suggests that TNA is a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for activity on a TNA template. We report that despite having a repeating unit that is one atom shorter than that of DNA, several polymerases showed surprisingly good ability to copy limited stretches of TNA. Copyright © 2003 American Chemical Society.
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页码：856 / 857
页数：2
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