Human Arf tumor suppressor specifically interacts with chromatin containing the promoter of rRNA genes

被引：40

作者：

Ayrault, O ^{[1
]}

Andrique, L ^{[1
]}

Larsen, CJ ^{[1
]}

Seite, P ^{[1
]}

机构：

[1] Lab Oncol Mol EA 3805, F-86022 Poitiers, France

来源：

ONCOGENE | 2004年 / 23卷 / 49期

关键词：

p14(ARF); Topo I; chromatin; ChIP; rRNA genes;

D O I：

10.1038/sj.onc.1207968

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tumor suppressor Arf (Alternative Reading Frame) protein (p14(ARF) in human and p19(ARF) in mouse) is mainly located in the nucleolus consistent with its subcellular localization, the protein has been shown to specifically interact with 5.8S rRNA and with B23/Nucleophosmin and to regulate ribosome biogenesis. Here, we show that the p14(ARF) protein interacts with chromatin and is recovered by chromatin immunoprecipitation (ChIP) in a fraction that contains a DNA sequence of the rRNA gene promoter. In addition, topoisomerase I (Topo I) that has been shown to interact with p14(ARF) coprecipitates with p14(ARF) containing chromatin. These data, in view of the function for Topo I in rRNA transcription, are consistent with a role for the p14(ARF)-Topo I complex in rRNA transcription and/or maturation.

引用

收藏

页码：8097 / 8104

页数：8

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