Roles of electro-acupuncture in glucose metabolism as assessed by 18F-FDG/PET imaging and AMPKα phosphorylation in rats with ischemic stroke

被引：38

作者：

Wu, Jingsong ^{[1
]}

Lin, Bingbing ^{[2
]}

Liu, Weilin ^{[1
]}

Huang, Jia ^{[1
]}

Shang, Guanhao ^{[2
]}

Lin, Yunjiao ^{[2
]}

Wang, Lulu ^{[2
]}

Chen, Lidian ^{[1
]}

Tao, Jing ^{[1
]}

机构：

[1] Fujian Univ Tradit Chinese Med, Coll Rehabil Med, Fuzhou 350122, Fujian, Peoples R China

[2] Fujian Key Lab Rehabil Technol, Fuzhou 350001, Fujian, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2017年 / 40卷 / 03期

基金：

中国国家自然科学基金;

关键词：

ischemic stroke; electro-acupuncture; glucose metabolism; positron emission tomography; AMP-activated protein kinase alpha; ACTIVATED PROTEIN-KINASE; CEREBRAL-ARTERY OCCLUSION; POSITRON-EMISSION-TOMOGRAPHY; REPERFUSION INJURED RATS; SIGNALING PATHWAY; PROVIDES NEUROPROTECTION; ADENOSINE-MONOPHOSPHATE; BRAIN-INJURY; IN-VIVO; MODEL;

D O I：

10.3892/ijmm.2017.3057

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Targeted energy metabolism balance contributes to neural survival during ischemic stroke. Herein, we tested the hypothesis that electro-acupuncture (EA) can enhance cerebral glucose metabolism assessed by F-18-fluorodeoxyglucose/positron emission tomography (F-18-FDG/PET) imaging to prevent propagation of tissue damage and improve neurological outcome in rats subjected to ischemia and reperfusion injury. Rats underwent middle cerebral artery occlusion (MCAO) and received EA treatment at the LI11 and ST36 acupoints or non-acupoint treatment once a day for 7 days. After EA treatment, a significant reduction in the infarct volume was determined by T2-weighted imaging, accompanied by the functional recovery in CatWalk and Rota-rod performance. Moreover, EA promoted higher glucose metabolism in the caudate putamen (CPu), motor cortex (MCTX), somatosensory cortex (SCTX) regions as assessed by animal F-18-FDG/PET imaging, suggesting that three-brain regional neural activity was enhanced by EA. In addition, the AMP-activated protein kinase alpha (AMPK alpha) in the CPu, MCTX and SCTX regions was phosphorylated at threonine 172 (Thr172) after ischemic injury; however, phosphorylation of AMPK was further increased by EA. These results indicate that EA could promote AMPK alpha phosphorylation of the CPu, MCTX and SCTX regions to enhance neural activity and motor functional recovery after ischemic stroke.

引用

页码：875 / 882

页数：8