Neuronal NF1/RAS regulation of cyclic AMP requires atypical PKC activation

被引:61
作者
Anastasaki, Corina [1 ]
Gutmann, David H. [1 ]
机构
[1] Washington Univ, Sch Med, Dept Neurol, St Louis, MO 63110 USA
关键词
PROTEIN-KINASE-C; PLURIPOTENT STEM-CELLS; NEUROFIBROMATOSIS TYPE-1; ADENYLYL-CYCLASE; RECEPTOR KINASE; MOUSE MODEL; MEK INHIBITION; IN-VITRO; RAS; DROSOPHILA;
D O I
10.1093/hmg/ddu389
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Neurofibromatosis type 1 (NF1) is a common neurodevelopmental disorder in which affected individuals are prone to learning, attention and behavioral problems. Previous studies in mice and flies have yielded conflicting results regarding the specific effector pathways responsible for NF1 protein (neurofibromin) regulation of neuronal function, with both cyclic AMP (cAMP)- and RAS-dependent mechanisms described. Herein, we leverage a combination of induced pluripotent stem cell-derived NF1 patient neural progenitor cells and Nf1 genetically engineered mice to establish, for the first time, that neurofibromin regulation of cAMP requires RAS activation in human and mouse neurons. However, instead of involving RAS-mediated MEK/AKT signaling, RAS regulation of cAMP homeostasis operates through the activation of atypical protein kinase C zeta, leading to GRK2-driven G alpha(s) inactivation. These findings reveal a novel mechanism by which RAS can regulate cAMP levels in the mammalian brain.
引用
收藏
页码:6712 / 6721
页数:10
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