Overexpression of archaeal proteins in Escherichia coli

被引：136

作者：

Kim, R

Sandler, SJ

Goldman, S

Yokota, H

Clark, AJ

Kim, SH ^{[1
]}

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Dept Biol Struct, Phys Biosci Div, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

BIOTECHNOLOGY LETTERS | 1998年 / 20卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1023/A:1005305330517

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Six archaeal proteins containing a high number of Escherichia coli rare codons in their genes were not well expressed in E. coli. These genes showed a five to twenty-fold increase in production when expressed in the presence of a plasmid harboring and expressing the argU and ileX genes encoding rare tRNAs(AGA/AGG)(arg) and tRNA(AUA)(ile).

引用

页码：207 / 210

页数：4

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