Overexpression of archaeal proteins in Escherichia coli

被引：136
|
作者：
Kim, R
Sandler, SJ
Goldman, S
Yokota, H
Clark, AJ
Kim, SH [1 ]
机构：
[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Dept Biol Struct, Phys Biosci Div, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
来源：
BIOTECHNOLOGY LETTERS | 1998年 / 20卷 / 03期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1023/A:1005305330517
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Six archaeal proteins containing a high number of Escherichia coli rare codons in their genes were not well expressed in E. coli. These genes showed a five to twenty-fold increase in production when expressed in the presence of a plasmid harboring and expressing the argU and ileX genes encoding rare tRNAs(AGA/AGG)(arg) and tRNA(AUA)(ile).
引用
收藏
页码：207 / 210
页数：4
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