The BEACH protein LvsB is localized on lysosomes and postlysosomes and limits their fusion with early endosomes

被引:37
作者
Kypri, Elena
Schmauch, Christian
Maniak, Markus
De Lozanne, Arturo [1 ]
机构
[1] Univ Texas, Inst Mol & Cellular Biol, Sect Mol Cell & Dev Biol, Austin, TX 78712 USA
[2] Univ Kassel, Abt Zellbiol, D-34132 Kassel, Germany
关键词
beige; CHS; Lyst;
D O I
10.1111/j.1600-0854.2007.00567.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The Chediak-Higashi syndrome (CHS) is a genetic disorder caused by the loss of the BEACH protein Lyst. Impaired lysosomal function in CHS patients results in many physiological problems, including immunodeficiency, albinism and neurological problems. Dictyostelium LvsB is the ortholog of mammalian Lyst and is also important for lysosomal function. A knock-in approach was used to tag LvsB with green fluorescent protein (GFP) and express it from its single chromosomal locus. GFP-LvsB was observed on late lysosomes and postlysosomes. Loss of LvsB resulted in enlarged postlysosomes, in the abnormal localization of proton pumps on postlysosomes and their abnormal acidification. The abnormal postlysosomes in LvsB-null cells were produced by the inappropriate fusion of early endosomal compartments with postlysosomal compartments. The intermixing of compartments resulted in a delayed transit of fluid-phase marker through the endolysosomal system. These results support the model that LvsB and Lyst proteins act as negative regulators of fusion by limiting the heterotypic fusion of early endosomes with postlysosomal compartments.
引用
收藏
页码:774 / 783
页数:10
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