Nitrite inhibition and intermediates effects on Anammox bacteria: A batch-scale experimental study

被引:106
作者
Bettazzi, Elena [1 ]
Caffaz, Simone [2 ]
Vannini, Claudia [3 ]
Lubello, Claudio [1 ]
机构
[1] Univ Florence, Dept Civil & Environm Engn, I-50139 Florence, Italy
[2] Publiacqua SpA, I-59126 Florence, Italy
[3] Univ Pisa, Dept Biol, I-56126 Pisa, Italy
关键词
Anammox; Inhibition; Intermediate compounds; Batch tests; Maximum activity; Manometric tests; ANAEROBIC AMMONIUM OXIDATION; IN-SITU DETECTION; AUTOTROPHIC NITROGEN REMOVAL; FLUIDIZED-BED REACTOR; OXIDIZING BACTERIA; START-UP; ENRICHMENT; WASTE; SLUDGE; WATER;
D O I
10.1016/j.procbio.2009.12.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A batch test procedure, based on manometric measurements, was used to study the Anammox process, in particular the inhibition due to nitrite and the effects of hydroxylamine and hydrazine, indicated as possible intermediates of the process. The maximum nitrite removal rate (MNRR) was measured. The method showed good reliability with a standard error of 4.5 +/- 3.3% (n: 41). All the tests were carried out on samples taken from a pilot plant with Anammox suspended biomass. The tests were used also to monitor the reactor activity. By testing different spiked additions of nitrite (10-75 mg NO(2)(-)-N L(-1)), a short-term inhibition, with more than 25% MNRR decrease, was found at concentrations higher than 60 mg NO(2)(-)-N L(-1). Repeated additions of nitrite higher than 30 mg NO(2)(-)-N L(-1) caused losses of activity. After a complete loss of activity, spiked additions of hydroxylamine (30 mg N L(-1) in total) determined a 20% permanent recovery. Low amounts of the intermediates (1-3 mg N L(-1)) applied on partially inhibited samples and uninhibited samples produced temporary increases in activity up to 50% and 30%, respectively. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:573 / 580
页数:8
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