Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods

被引:80
作者
Chen, Yanni [1 ,2 ]
Liu, Liqiang [1 ,2 ]
Xu, Liguang [1 ,2 ]
Song, Shanshan [1 ,2 ]
Kuang, Hua [1 ,2 ]
Cui, Gang [1 ,2 ]
Xu, Chuanlai [1 ,2 ]
机构
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China
基金
中国国家自然科学基金;
关键词
sulfonamides; gold immunochromatographic sensor; monoclonal antibody; honey samples; LINKED-IMMUNOSORBENT-ASSAY; TANDEM MASS-SPECTROMETRY; MULTIPLE DETECTION; PAPER SENSOR; IMMUNOASSAY; RESIDUES; STRIP; MILK; SULFAMETHAZINE; VALIDATION;
D O I
10.1007/s12274-017-1490-x
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A gold immunochromatographic sensor (GICS) was developed for the rapid detection of 26 sulfonamides in honey samples. The sensor was based on a group-specific monoclonal antibody (mAb) that can recognize all 26 sulfonamides. Three haptens (hapten 1 with a thiazole ring, hapten 2 with a benzene ring, and hapten 3 with a straight carbon chain) were used for antigen preparation. With hybridoma technology, a group-specific mAb was screened with a 50% maximal inhibitory concentration (IC50) against sulfathizole (STZ) and the other 25 analogues ranging from 0.08 to 90.18 ng/mL. Mono-dispersed gold nanoparticles were conjugated with the mAb to develop the lateral immunochromatographic strip. A labeled antibody concentration of 0.1 mu g/mL and a coating antigen concentration of 0.2 mu g/mL in the test line were chosen for strip preparation. Under optimized conditions, the visual limits of detection (vLOD) for the concentrations of STZ, sulfamethoxazole, sulfamethizole, sulfadiazine, sulfamerazine, sulfadimethoxine, sulfamonomethoxine, sulfameter, sulfamethoxypyridazine, and sulfachloropyridazine were 5, 0.25, 0.25, 10, 5, 10, 25, 2.5, 5, 0.25, and 10 mu g/kg, respectively. Scanner analysis in honey samples revealed good performance for detection of the 26 sulfonamides. Commercial honey samples were tested with the sensor and positive results were confirmed with high-performance liquid chromatography. The proposed strip sensor provides a convenient method for the rapid and reliable determination of sulfonamides pollutants in honey samples.
引用
收藏
页码:2833 / 2844
页数:12
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