Novel disease resistance specificities result from sequence exchange between tandemly repeated genes at the Cf-4/9 locus of tomato

被引：487

作者：

Parniske, M ^{[1
]}

HammondKosack, KE ^{[1
]}

Golstein, C ^{[1
]}

Thomas, CM ^{[1
]}

Jones, DA ^{[1
]}

Harrison, K ^{[1
]}

Wulff, BBH ^{[1
]}

Jones, JDG ^{[1
]}

机构：

[1] JOHN INNES CTR PLANT SCI RES,SAINSBURY LAB,NORWICH NR4 7UH,NORFOLK,ENGLAND

来源：

CELL | 1997年 / 91卷 / 06期

关键词：

D O I：

10.1016/S0092-8674(00)80470-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tomato Cf genes confer resistance to C. fulvum, reside in complex loci carrying multiple genes, and encode predicted membrane-bound proteins with extracytoplasmic leucine-rich repeats. At least two Cf-9 homologs confer novel C. fulvum resistance specificities. Comparison of 11 genes revealed 7 hypervariable amino acid positions in a motif of the leucine-rich repeats predicted to form a beta-strand/beta-turn in which the hypervariable residues are solvent exposed and potentially contribute to recognition specificity. Higher nonsynonymous than synonymous substitution rates in this region imply selection for sequence diversification. We propose that the level of polymorphism between intergenic regions determines the frequency of sequence exchange between the tandemly repeated genes. This permits sufficient exchange to generate sequence diversity but prevents sequence homogenization.

引用

页码：821 / 832

页数：12

共 40 条

[1] Molecular analysis of irradiation induced and spontaneous deletion mutants at a disease resistance locus in Lactuca sativa [J].

Anderson, PA ;

Okubara, PA ;

ArroyoGarcia, R ;

Meyers, BC ;

Michelmore, RW .

MOLECULAR & GENERAL GENETICS, 1996, 251 (03) :316-325

[2] Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region [J].

Anderson, PA ;

Lawrence, GJ ;

Morrish, BC ;

Ayliffe, MA ;

Finnegan, EJ ;

Ellis, JG .

PLANT CELL, 1997, 9 (04) :641-651

[3] GENERATION OF MHC CLASS-II DIVERSITY BY INTRAGENIC AND INTERGENIC RECOMBINATION [J].

ANDERSSON, L ;

MIKKO, S .

IMMUNOLOGICAL REVIEWS, 1995, 143 :5-12

[4] RFLP LINKAGE ANALYSIS OF THE CF-4 AND CF-9 GENES FOR RESISTANCE TO CLADOSPORIUM-FULVUM IN TOMATO [J].

BALINTKURTI, PJ ;

DIXON, MS ;

JONES, DA ;

NORCOTT, KA ;

JONES, JDG .

THEORETICAL AND APPLIED GENETICS, 1994, 88 (6-7) :691-700

[5]

BOTELLA MA, 1997, IN PRESS PLANT J

[6]

Boyes DC, 1997, PLANT CELL, V9, P237, DOI 10.1105/tpc.9.2.237

[7] The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins [J].

Dixon, MS ;

Jones, DA ;

Keddie, JS ;

Thomas, CM ;

Harrison, K ;

Jones, JDG .

CELL, 1996, 84 (03) :451-459

[8] Recombination occurs uniformly within the bronze gene, a meiotic recombination hotspot in the maize genome [J].

Dooner, HK ;

MartinezFerez, IM .

PLANT CELL, 1997, 9 (09) :1633-1646

[9] DETECTION AND QUANTIFICATION OF CONCERTED EVOLUTION AND MOLECULAR DRIVE [J].

DOVER, GA ;

LINARES, AR ;

BOWEN, T ;

HANCOCK, JM .

MOLECULAR EVOLUTION: PRODUCING THE BIOCHEMICAL DATA, 1993, 224 :525-541

[10] THE MATING-TYPE LOCUS OF CHLAMYDOMONAS-REINHARDTII CONTAINS HIGHLY REARRANGED DNA-SEQUENCES [J].

FERRIS, PJ ;

GOODENOUGH, UW .

CELL, 1994, 76 (06) :1135-1145

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