EMS-induced lincomycin resistance in red pepper (Capsicum annuum L.)

被引：0
作者：
Subhash, K [1 ]
Venkataiah, P [1 ]
Bhaskar, P [1 ]
机构：
[1] Kakatiya Univ, Dept Bot, Mutat Breeding & Tissue Culture Lab, Warangal 506009, Andhra Pradesh, India
来源：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 1997年 / 33卷 / 04期
关键词：
Capsicum annuum L; ethyl methane sulphonate; mutagenesis; maternal inheritance;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Ethyl methane sulphonate (EMS) is a potential mutagen to induce lincomycin resistance in Capsicum annuum. Mutagenized cotyledons were cultured on shoot regenerating medium containing lincomycin (100 mgl(-1)). Approximately 14% of regenerated shoots were chlorophyll deficient and about 4% of regenerated shoots were green from mutaganized cotyledons. The regenerated green plants were resistant to lincomycin but sensitive to chloramphenicol, kanamycin, spectinomycin, and streptomycin. Reciprocal crosses were made between resistant and sensitive plants. Inheritance of lincomycin resistance was transmitted as a non-Mendelian trait. Lincomycin resistance is a first selectable and maternally inherited organelle encoded genetic marker described in chili pepper. Such mutants should be useful in designing biochemical selection schemes to recover somatic hybrids and cybrids.
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页码：285 / 287
页数：3
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