Biochemical Methods to Monitor Autophagic Responses in Plants

被引：11

作者：

Bao, Y. ^{[1
]}

Mugume, Y. ^{[1
]}

Bassham, D. C. ^{[1
]}

机构：

[1] Iowa State Univ, Ames, IA 50011 USA

来源：

MOLECULAR CHARACTERIZATION OF AUTOPHAGIC RESPONSES, PT B | 2017年 / 588卷

基金：

美国国家科学基金会;

关键词：

SELECTIVE AUTOPHAGY; ENDOPLASMIC-RETICULUM; STRESS RESPONSES; CELL-DEATH; PROTEINS; DEGRADATION; ATG8; SENESCENCE; YEAST; CHLOROPLASTS;

D O I：

10.1016/bs.mie.2016.09.090

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The study of autophagy in plants is rapidly increasing, due to its pivotal and fundamental roles in responding to stressful stimuli, recycling nutrients during senescence, and maintaining growth under normal conditions. Assays for detecting autophagy in plants have generally been based on microscopic observations, providing qualitative information on autophagy activity. Here, we discuss biochemical assays for detecting autophagy, which have the potential for providing more quantitative information, with a focus on immunoblotting with antibodies against ATG8, NBR1, or epitope tags fused to ATG proteins.

引用

收藏

页码：497 / 513

页数：17

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