How to gather useful and valuable information from protein binding measurements using Langmuir lipid monolayers

被引：27

作者：

Boisselier, Elodie ^{[1
]}

Demers, Eric

Cantin, Line

Salesse, Christian ^{[1
]}

机构：

[1] CHU Quebec, Ctr Rech, Hop St Sacrement, CUO Rech, 1050 Chemin Ste Foy, Quebec City, PQ G1S 4L8, Canada

来源：

ADVANCES IN COLLOID AND INTERFACE SCIENCE | 2017年 / 243卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

Monolayer; Langmuir films; Protein binding; Retinitis pigmentosa 2; RP2; AIR-WATER-INTERFACE; INFRARED REFLECTION-ABSORPTION; HEATED LYSOZYME INTERACTIONS; OUTER SEGMENT PHOSPHOLIPIDS; BREWSTER-ANGLE MICROSCOPY; IN-SITU MEASUREMENT; N-TERMINAL SEGMENT; FT-IR SPECTROSCOPY; X-RAY REFLECTIVITY; II CORE COMPLEX;

D O I：

10.1016/j.cis.2017.03.004

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This review presents data on the influence of various experimental parameters on the binding of proteins onto Langmuir lipid monolayers. The users of the Langmuir methodology are often unaware of the importance of choosing appropriate experimental conditions to validate the data acquired with this method. The protein Retinitis pigmentosa 2 (RP2) has been used throughout this review to illustrate the influence of these experimental parameters on the data gathered with Langmuir monolayers. The methods detailed in this review include the determination of protein binding parameters from the measurement of adsorption isotherms, infrared spectra of the protein in solution and in monolayers, ellipsometric isotherms and fluorescence micrographs. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：60 / 76

页数：17

共 299 条

[1]

Ael S Abou El Ela, 2014, SAUDI PHARM J, V22, P555

[2] Interactions of the HIV-1 fusion peptide with large unilamellar vesicles and monolayers.: A cryo-TEM and spectroscopic study [J].