Design of a surface-functionalized power-free microchip for extracellular vesicle detection utilizing UV grafting

As a biomarker for various diseases, extracellular vesicles (EV) in bodily fluids have received attention. In this study, poly(2-aminoethyl methacrylate hydrochloride) (PAEMA) was grafted onto a poly(dimethylsiloxane) power-free microchip by UV light-induced graft polymerization and anti-CD 63 antibody for EV capture was immobilized. Thus, a surface-functionalized power-free microchip (SF-PF microchip), which enables simple and rapid EV detection was fabricated. There was a correlation between the amount of antibody immobilized and the EV detection signal intensity, and both the amount and the signal intensity showed a maximum at 0.5 mol/L of AEMA concentration in feed for grafting. The sample volume required for EV detection was 1.0 mu L and the time required for detection was 13 min. The SF-PF microchip would contribute to a point-of-care testing application.