Evaluation of a tunable bandpass reaction cell inductively coupled plasma mass spectrometer for the determination of selenium in serum and urine

A Dynamic Reaction Cell(TM) inductively coupled plasma mass spectrometer (DRC-ICP-MS) was evaluated for the determination of selenium in serum and urine. Reaction cell conditions were evaluated for the suppression of Ar-2(+) dimer at m/z 78 and 80 using methane as the reaction gas. A diluent containing 10% ethanol, 1% nitric acid, 0.5% Triton X-100 with gallium and yttrium internal standards was used to dilute urine and serum samples. Instrument response calibration was achieved by using aqueous acidic standards spiked into a urine matrix. Slopes for aqueous inorganic selenium, seleno-DL-cystine, seleno-DL-methionine and trimethylselenonium iodide spiked into urine and serum matrices were nearly identical. In general, reagent blank readings and detection limits were significantly lower in the DRC mode (reaction cell pressurized) than the standard mode (cell vented). Average results for the analysis of National Institute of Standards and Technology Standard Reference Material (NIST SRM) 1598 bovine serum (attained over 13 days) are: 43.8 +/- 3.6 mug Se/l. Reference concentration is 43.6 +/- 3.6 mug Se/l. For NIST SRM 2670 Normal Urine the DRC-ICP-MS results are 30.7 +/- 4.6 mug Se/l with a certified concentration of 30 +/- 8 mug Se/l. For NIST SRM 2670 Elevated Urine the DRC-ICP-MS results are 463 +/- 35 mug Se/l with a certified concentration of 460 +/- 30 mug Se/l. The DRC-ICP-MS results for selenium determinations in urine and serum survey samples from the Institut National de Saute Publique du Quebec were compared with the reference concentrations and results produced by conventional ICP-MS. While conventional ICP-MS gave acceptable results for survey samples, DRC-ICP-MS gave excellent results for both urine and sera. Closer correlation was observed for DRC-ICP-MS results with target concentrations than with conventional ICP-MS. (C) 2002 Elsevier Science B.V. All rights reserved.