Morin attenuates pyroptosis of nucleus pulposus cells and ameliorates intervertebral disc degeneration via inhibition of the TXNIP/NLRP3/Caspase-1/IL-1β signaling pathway

被引：57

作者：

Zhou, Yifan ^{[1
]}

Chen, Zhiqian ^{[1
]}

Yang, Xiao ^{[1
]}

Cao, Xiankun ^{[1
]}

Liang, Zhihao ^{[1
]}

Ma, Hui ^{[1
]}

Zhao, Jie ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthopaed Surg, Shanghai Key Lab Orthopaed Implants,Sch Med, Shanghai, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2021年 / 559卷

基金：

中国国家自然科学基金;

关键词：

Intervertebral disc degeneration; Morin; Pyroptosis; TXNIP;

D O I：

10.1016/j.bbrc.2021.04.090

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intervertebral disc degeneration (IDD) is a major cause of lower back pain (LBP), a condition that causes a heavy economic burden globally. The production of cytokines, including interleukin (IL)-10 and tumor necrosis factor (TNF) a, is increased in the degenerating intervertebral disc. Thioredoxin-interacting protein (TXNIP) participates in NLRP3 inflammasome-dependent pyroptosis in liver. Therefore, we hypothesized that TXNIP maypromote pyroptosis via NLRP3/Caspase-1/IL-10 signaling pathway in nucleus pulposus (NP) cell. This study examined the effects of TXNIP on IDD, explored the underlying mechanisms of action and find Morin which is the inhibitor of TXNIP can attenuates pyroptosis of nucleus pulposus cells and ameliorates intervertebral disc degeneration. Our findings indicate that TXNIP promote pyroptosis via NLRP3/Caspase-1/IL-10 signaling pathway in NP cell. Morin considerably inhibited the TXNIP/NLRP3/Caspase-1 signaling pathway in vitro. In vivo. Our data show that TXNIP can aggravates intervertebral disc degeneration and morin may be a useful therapeutic agent for IDD. (c) 2021 Elsevier Inc. All rights reserved.

引用

页码：106 / 112

页数：7

共 26 条

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