Expression, Purification and Characterization of C-FADD

被引:6
作者
Chen, Yuan [1 ,2 ]
Ma, Dingyuan [1 ,2 ]
Huang, Qi-Lai [1 ,2 ]
Zheng, Weijuan [1 ,2 ]
Zhang, Jing [1 ,2 ]
Shen, Yi [1 ,2 ]
Li, Jiahuang [1 ,2 ]
Dong, Wei [1 ,2 ]
Lu, Min [1 ,2 ]
Wang, Jin [1 ,2 ]
Hua, Zi-Chun [1 ,2 ,3 ]
机构
[1] Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China
[2] Nanjing Univ, Jiangsu Ctr Hepatobiliary Dis, Coll Life Sci, Nanjing 210093, Peoples R China
[3] Nanjing Univ, Changzhou High Tech Res Inst, Changzhou 213164, Peoples R China
关键词
C-FADD; expression; purification; monomer; DEATH DOMAIN PROTEIN; INHIBITS PROLIFERATION; FAS; PHOSPHORYLATION; FADD/MORT1; ACTIVATION; INTERACTS; MUTANT; MICE;
D O I
10.1038/cmi.2009.39
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
FADD is an important proapoptotic adaptor in death receptor-induced apoptosis. Recently, FADD has been found to participate in a variety of non-apoptotic processes, such as development, cell cycle progression and survival. Its non-apoptotic activities were regulated by the phosphorylated status of the serine residue located at the C-terminal region, a domain distinct from the proapoptotic function related DED and DD domains. However, due to the difficulties in expression and crystallization of natural FADD, by far the molecular structures of all FADD variants did not contain the C-terminal region. To elucidate the structure-function relationship of C-terminal region, we need to obtain a FADD variant that containing C-terminal region. In this study, mouse FADD (80-205) containing DD domain and C-terminal region, designated as C-FADD, was expressed in E. coli with His-tag at the N-terminus and purified by Ni2+ affinity chromatography. The purified protein existed as a homogenous monomer in glutaraldehyde cross-linking analysis and exhibited a typical a-helix spectrum in. CD (circular dichroism) assay. In vitro His-tag pull-down assay demonstrated that the purified C-FADD possessed the CK I alpha-binding activity which was important for its non-apoptotic function. Cellular & Molecular Immunology. 2009;6(4):295-301.
引用
收藏
页码:295 / 301
页数:7
相关论文
共 31 条
[1]   Phosphorylation of FADD at serine 194 by CKIα regulates its nonapoptotic activities [J].
Alappat, EC ;
Feig, C ;
Boyerinas, B ;
Volkland, J ;
Samuels, M ;
Murmann, AE ;
Thorburn, A ;
Kidd, VJ ;
Slaughter, CA ;
Osborn, SL ;
Winoto, A ;
Tang, WJ ;
Peter, ME .
MOLECULAR CELL, 2005, 19 (03) :321-332
[2]   Cell cycle effects by C-FADD depend on its C-terminal phosphorylation site [J].
Alappat, EC ;
Volkland, J ;
Peter, ME .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (43) :41585-41588
[3]   Death receptors: Signaling and modulation [J].
Ashkenazi, A ;
Dixit, VM .
SCIENCE, 1998, 281 (5381) :1305-1308
[4]   The death effector domain protein family [J].
Barnhart, BC ;
Lee, JC ;
Alappat, EC ;
Peter, ME .
ONCOGENE, 2003, 22 (53) :8634-8644
[5]   The three-dimensional solution structure and dynamic properties of the human FADD death domain [J].
Berglund, H ;
Olerenshaw, D ;
Sankar, A ;
Federwisch, M ;
McDonald, NQ ;
Driscoll, PC .
JOURNAL OF MOLECULAR BIOLOGY, 2000, 302 (01) :171-188
[6]   A NOVEL PROTEIN THAT INTERACTS WITH THE DEATH DOMAIN OF FAS/APO1 CONTAINS A SEQUENCE MOTIF RELATED TO THE DEATH DOMAIN [J].
BOLDIN, MP ;
VARFOLOMEEV, EE ;
PANCER, Z ;
METT, IL ;
CAMONIS, JH ;
WALLACH, D .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (14) :7795-7798
[7]   The structure of FADD and its mode of interaction with procaspase-8 [J].
Carrington, Paul E. ;
Sandu, Cristinel ;
Wei, Yufeng ;
Hill, Justine M. ;
Morisawa, Gaku ;
Huang, Ted ;
Gavathiotis, Evridipis ;
Wei, Yu ;
Werner, Milton H. .
MOLECULAR CELL, 2006, 22 (05) :599-610
[8]   Immobilized protein ZZ, an affinity tool for immunoglobulin isolation and immunological experimentation [J].
Chen, Cheng ;
Huang, Qi-Lai ;
Jiang, Shu-Han ;
Pan, Xiao ;
Hua, Zi-Chun .
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2006, 45 :87-92
[9]   FADD, A NOVEL DEATH DOMAIN-CONTAINING PROTEIN, INTERACTS WITH THE DEATH DOMAIN OF FAS AND INITIATES APOPTOSIS [J].
CHINNAIYAN, AM ;
OROURKE, K ;
TEWARI, M ;
DIXIT, VM .
CELL, 1995, 81 (04) :505-512
[10]   Signal transduction by DR3, a death domain-containing receptor related to TNFR-1 and CD95 [J].
Chinnaiyan, AM ;
ORourke, K ;
Yu, GL ;
Lyons, RH ;
Garg, M ;
Duan, DR ;
Xing, L ;
Gentz, R ;
Ni, J ;
Dixit, VM .
SCIENCE, 1996, 274 (5289) :990-992