VirB/D4-dependent protein translocation from Agrobacterium into plant cells

被引：300

作者：

Vergunst, AC ^{[1
]}

Schrammeijer, B ^{[1
]}

den Dulk-Ras, A ^{[1
]}

de Vlaam, CMT ^{[1
]}

Regensburg-Tuïnk, TJG ^{[1
]}

Hooykaas, PJJ ^{[1
]}

机构：

[1] Leiden State Univ, Inst Mol Plant Sci, Clusius Lab, NL-2333 AL Leiden, Netherlands

来源：

SCIENCE | 2000年 / 290卷 / 5493期

关键词：

D O I：

10.1126/science.290.5493.979

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Agrobacterium VirB/D4 transport system mediates the transfer of a nucleoprotein T complex into plant cells, Leading to crown gall disease. In addition, several Virulence proteins must somehow be transported to fulfill a function in planta. Here, we used fusions between Cre recombinase and VirE2 or VirF to directly demonstrate protein translocation into plant cells. Transport of the proteins was monitored by a Cre-mediated in planta recombination event resulting in a selectable phenotype and depended on the VirB/D4 transport system but did not require transferred DNA.

引用

页码：979 / 982

页数：4

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