Characterization of novel SUMO family genes in the rice genome

被引：6

作者：

Teramura, Hiroshi ^{[1
]}

Yamada, Kazuma ^{[1
]}

Ito, Kahori ^{[1
]}

Kasahara, Keisuke ^{[1
]}

Kikuchi, Tsubasa ^{[1
]}

Kioka, Naoya ^{[1
]}

Fukuda, Masato ^{[1
]}

Kusano, Hiroaki ^{[1
,3
]}

Tanaka, Katsunori ^{[2
]}

Shimada, Hiroaki ^{[1
]}

机构：

[1] Tokyo Univ Sci, Dept Biol Sci & Technol, Katsushika, Tokyo 1258585, Japan

[2] Kwansei Gakuin Univ, Sch Sci & Technol, Dept Biosci, Sanda, Hyogo 6691337, Japan

[3] Kyoto Univ, Res Inst Sustainable Humanosphere, Uji, Kyoto 6110011, Japan

来源：

GENES & GENETIC SYSTEMS | 2021年 / 96卷 / 01期

关键词：

complementation of fission yeast SUMO mutant; SUMOylation using the bacterial expression system; post-translational modification; rice; PROTEIN MODIFICATION; MODIFIER SUMO; UBIQUITIN; ARABIDOPSIS; SUMOYLATION; SYSTEM; PHOSPHATE; RESPONSES; INSIGHTS;

D O I：

10.1266/ggs.20-00034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Small ubiquitin-related modifier (SUMO) is a post-translational modification factor composed of about 100 amino acid residues. Most plant species express a family of SUMO isoforms. We found three novel homologs of rice (Oryza sativa L.) SUMO genes, OsSUMO4, OsSUMO5 and OsSUMO6, in addition to the known SUMO genes OsSUMO1-OsSUMO3. Phylogenetic tree analysis revealed that rice SUMO genes have diverged considerably during their evolution. All six of these SUMO genes complemented the phenotype of the SUMO-deficient pmt3 Delta mutant of fission yeast. Among the amino acid sequences of rice SUMO proteins, consensus motifs (psi KXE/D) of the SUMO acceptor site were found in OsSUMO3, OsSUMO4, OsSUMO5 and OsSUMO6. The heat shock protein HSF7 is known to be SUMOylated in Arabidopsis thaliana. SUMOylation using a bacterial expression system revealed that the rice HSF7 homolog was modified by the six rice SUMOs, and further suggested that OsSUMO1, OsSUMO3, OsSUMO4 and OsSUMO6 are involved in its multiple SUMOylation.

引用

页码：25 / 32

页数：8

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