DNA Photo-Cross-Linking Using 3-Cyanovinylcarbazole Modified Oligonucleotide with Threoninol Linker

被引：35
|
作者：
Sakamoto, Takashi [1 ]
Tanaka, Yuya [1 ]
Fujimoto, Kenzo [1 ,2 ]
机构：
[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Nomi, Ishikawa 9231292, Japan
[2] Japan Adv Inst Sci & Technol, Res Ctr Bioarchitecture, Nomi, Ishikawa 9231292, Japan
来源：
ORGANIC LETTERS | 2015年 / 17卷 / 04期
关键词：
POINT MUTATIONS; DOUBLE-STRAND; RNA; METHYLPHOSPHONATES; NUCLEOSIDE; DERIVATIVES; TRANSITION; TRIPLEX; QUICK;
D O I：
10.1021/acs.orglett.5b00035
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
3-Cyanovinylcarbazole modified d-threoninol (D-CNV) was incorporated in oligodeoxyribonucleotide and tested for a photo-cross-linking reaction with complementary oligodeoxyribonucleotide. The photoreactivity was 1.8- to 8-fold greater than that of 3-cyanovinylcarbazole modified deoxyribose (K-CNV) previously reported. From the results of melting analysis and circular dichroism spectroscopy of the duplexes, the relatively flexible structure of D-CNV compared with K-CNV might be advantageous for [2 + 2] photocycloaddition between the cyanovinyl group on the D-CNV and pyrimidine base in the complementary strand.
引用
收藏
页码：936 / 939
页数：4
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