Clinical two-photon microendoscopy

被引：146

作者：

Koenig, K. ^{[1
]}

Ehlers, A.

Riemann, I.

Schenkl, S.

Bueckle, R.

Kaatz, M.

机构：

[1] Fraunhofer Inst Biomed Technol, St Ingbert, Saarland, Germany

[2] Univ Saarland, Fac Phys & Mechatron, D-6600 Saarbrucken, Saarland, Germany

[3] JenLab GmbH, Jena, Thuringia, Germany

[4] Univ Jena, Dept Dermatol, D-6900 Jena, Thuringia, Germany

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2007年 / 70卷 / 05期

关键词：

endoscopy; multiphoton; tomography; DermaInspect; GRIN; SHG;

D O I：

10.1002/jemt.20445

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Two-photon medical imaging has found its way into dermatology as an excellent method for noninvasive skin cancer detection without need of contrast agents as well as for in situ drug screening of topically-applied cosmetical and pharmaceutical components. There is an increasing demand to apply the multiphoton technology also for deep-tissue skin imaging as well as for intracorporal imaging. We report on the first clinical use of multiphoton endoscopes, in particular of a miniaturized rigid two-photon GRIN lens endoscope. The microendoscope was attached to the multiphoton tomograph DermaInspect and employed to detect the extracellular matrix proteins collagen and elastin in the human dermis of volunteers and patients with ulcera by in vivo second harmonic generation and in vivo two-photon autofluorescence.

引用

页码：398 / 402

页数：5