The use of genome sequencing to investigate an outbreak of hospital-acquired mucormycosis in transplant patients

被引：7
作者：
Marek, Caitlyn [1 ]
Croxen, Matthew A. [1 ,2 ]
Dingle, Tanis C. [1 ,2 ]
Bharat, Amrita [3 ]
Schwartz, Ilan S. [1 ]
Wiens, Rhoda [4 ]
Smith, Stephanie [1 ]
机构：
[1] Univ Alberta, Edmonton, AB, Canada
[2] Publ Hlth Lab, Edmonton, AB, Canada
[3] Publ Hlth Agcy Canada, Natl Microbiol Lab, Winnipeg, MB, Canada
[4] Univ Alberta, Alberta Hlth Serv, Edmonton, AB, Canada
来源：
TRANSPLANT INFECTIOUS DISEASE | 2019年 / 21卷 / 05期
关键词：
disease outbreaks; infection control; Mucorales; mucormycosis; Rhizomucor; whole genome sequencing;
D O I：
10.1111/tid.13163
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We report three cases of hospital-acquired mucormycosis in heart and lung transplant patients over a 6-month period. Traditional epidemiological investigation tools were used to look for a common link between patients to explain the outbreak. Genome sequencing of each fungal strain was used to supplement the investigation. By disproving a close genetic link between infecting strains of mucormycosis, we were able to conclude the outbreak investigation. Genome sequencing is a novel tool that can be used in addition to traditional epidemiologic investigations to help determine linkage of patients during outbreak investigations.
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页数：4
相关论文
共 16 条
  • [1] SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
    Bankevich, Anton
    Nurk, Sergey
    Antipov, Dmitry
    Gurevich, Alexey A.
    Dvorkin, Mikhail
    Kulikov, Alexander S.
    Lesin, Valery M.
    Nikolenko, Sergey I.
    Son Pham
    Prjibelski, Andrey D.
    Pyshkin, Alexey V.
    Sirotkin, Alexander V.
    Vyahhi, Nikolay
    Tesler, Glenn
    Alekseyev, Max A.
    Pevzner, Pavel A.
    [J]. JOURNAL OF COMPUTATIONAL BIOLOGY, 2012, 19 (05) : 455 - 477
  • [2] Fungal biodiversity - as found in nasal mucus
    Buzina, W
    Braun, H
    Freudenschuss, K
    Lackner, A
    Habermann, W
    Stammberger, H
    [J]. MEDICAL MYCOLOGY, 2003, 41 (02) : 149 - 161
  • [3] Healthcare-associated outbreaks due to Mucorales and other uncommon fungi
    Davoudi, Setareh
    Graviss, Linda S.
    Kontoyiannis, Dimitrios P.
    [J]. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 2015, 45 (07) : 767 - 773
  • [4] Mucormycosis Outbreak Associated With Hospital Linens
    Duffy, Jonathan
    Harris, Julie
    Gade, Lalitha
    Sehulster, Lynne
    Newhouse, Emily
    O'Connell, Heather
    Noble-Wang, Judith
    Rao, Carol
    Balajee, S. Arunmozhi
    Chiller, Tom
    [J]. PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2014, 33 (05) : 472 - 476
  • [5] Garcia-Hermoso D, 2018, MBIO, V9, DOI [10.1128/mBio.00573-18, 10.1128/mbio.00573-18]
  • [6] Katz LS, 2017, AM SOC MICR C RAP AP
  • [7] Novosad SA, 2016, MMWR-MORBID MORTAL W, V65, P481, DOI 10.15585/mmwr.mm6518a5
  • [8] Epidemiology and Clinical Manifestations of Mucormycosis
    Petrikkos, George
    Skiada, Anna
    Lortholary, Olivier
    Roilides, Emmanuel
    Walsh, Thomas J.
    Kontoyiannis, Dimitrios P.
    [J]. CLINICAL INFECTIOUS DISEASES, 2012, 54 : S23 - S34
  • [9] Whole-Genome Sequencing of Bacterial Pathogens: the Future of Nosocomial Outbreak Analysis
    Quainoo, Scott
    Coolen, Jordy P. M.
    van Hijum, Sacha A. F. T.
    Huynen, Martijn A.
    Melchers, Willem J. G.
    van Schaik, Willem
    Wertheim, Heiman F. L.
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 2017, 30 (04) : 1015 - 1063
  • [10] Healthcare-Associated Mucormycosis
    Rammaert, Blandine
    Lanternier, Fanny
    Zahar, Jean-Ralph
    Dannaoui, Eric
    Bougnoux, Marie-Elisabeth
    Lecuit, Marc
    Lortholary, Olivier
    [J]. CLINICAL INFECTIOUS DISEASES, 2012, 54 : S44 - S54
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