A Real-Time PCR for Detection and Quantification of Mycoplasma ovipneumoniae

被引：18

作者：

Yang, Falong ^{[1
]}

Dao, Xiaofang ^{[1
]}

Rodriguez-Palacios, Alex ^{[2
]}

Feng, Xufei ^{[1
]}

Tang, Cheng ^{[1
]}

Yang, Xiaonong ^{[1
]}

Yue, Hua ^{[1
]}

机构：

[1] Southwest Univ Nationalities, Dept Vet Med, Coll Life Sci & Technol, Chengdu 610041, Peoples R China

[2] Case Western Reserve Univ, Div Gastrointestinal & Liver Dis, Cleveland, OH 44106 USA

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2014年 / 76卷 / 12期

关键词：

Mycoplasma ovipneumoniae; quantification; real-time PCR; POLYMERASE-CHAIN-REACTION; BIGHORN SHEEP; RESPIRATORY-DISEASE; REACTION ASSAY; SEQUENCE; AGALACTIAE; CULTURE; DNA;

D O I：

10.1292/jvms.14-0094

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A real-time PCR for detection and quantification of M ovipneumoniae was developed using 9 recently sequenced M ovipneumoniae genomes and primers targeting a putative adhesin gene p113. The assay proved to be specific and sensitive (with a detection limit of 22 genomic DNA) and could quantify M ovipneumoniae DNA over a wide linear range, from 2.2 x 10(2) to 2.2 x 10(7) genomes.

引用

页码：1631 / 1634

页数：4

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