Technology for high-throughput screens: the present and future using zebrafish

被引：87
作者：
Love, DR
Pichler, FB
Dodd, A
Copp, BR
Greenwood, DR
机构：
[1] Univ Auckland, Sch Biol Sci, Auckland 1001, New Zealand
[2] Univ Auckland, Dept Chem, Auckland 1001, New Zealand
[3] Gene Technol, HortRes, Mt Albert Res Ctr, Auckland 1003, New Zealand
来源：
CURRENT OPINION IN BIOTECHNOLOGY | 2004年 / 15卷 / 06期
关键词：
D O I：
10.1016/j.copbio.2004.09.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The zebrafish is a popular vertebrate model organism with similar organ systems and gene sequences to humans. Zebrafish embryos are optically transparent enabling organ visualisation, which can be complemented with gene expression analysis at the transcript and protein levels. Furthermore, zebrafish can be treated with small molecules and drugs in a microtitre plate format for high-throughput analysis and for the identification and validation of drugs. High-throughput methodologies for use in zebrafish include phenotype-based visualisation, transcript studies using low-density DNA microarrays and proteomic analysis. These technologies offer significant whole-organism biological value in the drug discovery and drug development pipeline.
引用
收藏
页码：564 / 571
页数：8
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