On-chip signal amplification of magnetic bead-based immunoassay by aviating magnetic bead chains

被引：17

作者：

Jalal, Uddin M. ^{[1
]}

Jin, Gyeong Jun ^{[1
]}

Eom, Kyu Shik ^{[1
]}

Kim, Min Ho ^{[1
]}

Shim, Joon S. ^{[1
]}

机构：

[1] Kwangwoon Univ, Dept Elect Convergence Engn, Bio IT Convergence Lab, Seoul, South Korea

来源：

BIOELECTROCHEMISTRY | 2018年 / 122卷

基金：

新加坡国家研究基金会;

关键词：

Magnetic bead; Lab-on-a-Chip (LOC); beta-hCG; Antibody; Immunoassay; HUMAN CHORIONIC-GONADOTROPIN; MICROPIPETTE TIP; CHORIOCARCINOMA; PREGNANCY; ACTUATION; TRANSPORT; HORMONE; CAPTURE; PROTEIN; SURFACE;

D O I：

10.1016/j.bioelechem.2017.11.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work, a Lab-on-a-Chip (LOC) platform is used to electromagnetically actuate magnetic bead chains for an enhanced immunoassay. Custom-made electromagnets generate a magnetic field to form, rotate, lift and lower the magnetic bead chains (MBCs). The cost-effective, disposable LOC platform was made with a polymer substrate and an on-chip electrochemical sensor patterned via the screen-printing process. The movement of the MBC5 is controlled to improve the electrochemical signal up to 230% when detecting beta-type human chorionic gonadotropin (beta-hCG). Thus, the proposed on-chip MBC-based immunoassay is applicable for rapid, qualitative electrochemical point-of-care (POC) analysis. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：221 / 226

页数：6

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