On-chip signal amplification of magnetic bead-based immunoassay by aviating magnetic bead chains

被引:17
作者
Jalal, Uddin M. [1 ]
Jin, Gyeong Jun [1 ]
Eom, Kyu Shik [1 ]
Kim, Min Ho [1 ]
Shim, Joon S. [1 ]
机构
[1] Kwangwoon Univ, Dept Elect Convergence Engn, Bio IT Convergence Lab, Seoul, South Korea
基金
新加坡国家研究基金会;
关键词
Magnetic bead; Lab-on-a-Chip (LOC); beta-hCG; Antibody; Immunoassay; HUMAN CHORIONIC-GONADOTROPIN; MICROPIPETTE TIP; CHORIOCARCINOMA; PREGNANCY; ACTUATION; TRANSPORT; HORMONE; CAPTURE; PROTEIN; SURFACE;
D O I
10.1016/j.bioelechem.2017.11.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this work, a Lab-on-a-Chip (LOC) platform is used to electromagnetically actuate magnetic bead chains for an enhanced immunoassay. Custom-made electromagnets generate a magnetic field to form, rotate, lift and lower the magnetic bead chains (MBCs). The cost-effective, disposable LOC platform was made with a polymer substrate and an on-chip electrochemical sensor patterned via the screen-printing process. The movement of the MBC5 is controlled to improve the electrochemical signal up to 230% when detecting beta-type human chorionic gonadotropin (beta-hCG). Thus, the proposed on-chip MBC-based immunoassay is applicable for rapid, qualitative electrochemical point-of-care (POC) analysis. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:221 / 226
页数:6
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