Overexpression of the Arabidopsis glutathione peroxidase-like 5 gene (AtGPXL5) resulted in altered plant development and redox status

被引:23
作者
Riyazuddin, Riyazuddin [1 ,2 ]
Bela, Krisztina [1 ]
Horvath, Edit [3 ]
Rigo, Gabor [1 ,3 ]
Galle, Agnes [1 ]
Szabados, Laszlo [3 ]
Feher, Attila [1 ,3 ]
Csiszar, Jolan [1 ]
机构
[1] Univ Szeged, Fac Sci & Informat, Dept Plant Biol, Kozep Fasor 52, H-6726 Szeged, Hungary
[2] Univ Szeged, Fac Sci & Informat, Doctoral Sch Biol, Szeged, Hungary
[3] Biol Res Ctr HAS, Inst Plant Biol, Temesvari Krt 62, H-6726 Szeged, Hungary
关键词
Antioxidant mechanisms; Arabidopsis thaliana; Glutathione peroxidase-like; Reactive oxygen species; Redox homeostasis; Salt stress; SALICYLIC-ACID; SALT TOLERANCE; ANTIOXIDANT ENZYMES; EXPRESSION ANALYSES; ROOT ARCHITECTURE; STRESS MARKER; ABSCISIC-ACID; THIOREDOXIN; IDENTIFICATION; HOMEOSTASIS;
D O I
10.1016/j.envexpbot.2019.103849
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant's glutathione peroxidase-like (GPXL) enzymes are thiol-based peroxidases catalysing the reduction of H2O2 or hydroperoxides to water or alcohols using reduced glutathione (GSH) or thioredoxin as an electron donor. Arabidopsis thaliana possess eight isoenzymes having different roles in redox-dependent processes. AtGPXL5 is a poorly known plasma membrane associated enzyme. We have investigated the role of AtGPXL5 in development and responses to salt using AtGPXL5-overexpressing lines (OX-AtGPXL5) and Atgpxl5 mutants. Constitutive overexpression of AtGPXL5 increased the transcription of the gene by 17-24 times in 6-week-old plants. In OX-AtGPXL5 plants, the activity of glutathione peroxidase, thioredoxin peroxidase and most of the main antioxidant enzymes were like in the wild type Col-0, but the amount of GSH was increased, thus the redox potential became more negative compared to the wild type. The well-preserved germination rate, seedling growth and chlorophyll content of the OX-AtGPXL5 seedlings in the presence of 100 mM NaCl indicated the increased salt tolerance of AtGPXL5-overexpressing plants. In agreement, the Atgpxl5 knockdown mutants had enhanced salt stress sensitivity in comparison to the wild type. Our results indicate that AtGPXL5 may have function in the fine-tuning of ROS levels and redox status during salt stress.
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页数:13
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