Inhibition of the FACT Complex Reduces Transcription from the Human Cytomegalovirus Major Immediate Early Promoter in Models of Lytic and Latent Replication

被引：23

作者：

O'Connor, Christine M. ^{[1
]}

Nukui, Masatoshi ^{[2
]}

Gurova, Katerina V. ^{[3
]}

Murphy, Eain A. ^{[2
]}

机构：

[1] SUNY Buffalo, Dept Microbiol & Immunol, Buffalo, NY 14260 USA

[2] FORGE Life Sci LLC, Baruch S Blumberg Res Inst, Doylestown, PA USA

[3] Roswell Pk Canc Inst, Dept Cell Stress Biol, Buffalo, NY 14263 USA

来源：

JOURNAL OF VIROLOGY | 2016年 / 90卷 / 08期

关键词：

HEMATOPOIETIC PROGENITOR CELLS; KAPPA-B PATHWAY; GENE-EXPRESSION; REACTIVATION; ACTIVATION; STIMULATION; ACETYLATION; FIBROBLASTS; INFECTION; ENHANCER;

D O I：

10.1128/JVI.02501-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The successful colonization of the majority of the population by human cytomegalovirus is a direct result of the virus's ability to establish and, more specifically, reactivate from latency. The underlying cellular factors involved in viral reactivation remain unknown. Here, we show that the host complex facilitates chromatin transcription ( FACT) binds to the major immediate early promoter ( MIEP) and that inhibition of this complex reduces MIEP transactivation, thus inhibiting viral reactivation.

引用

页码：4249 / 4253

页数：5

共 21 条

[1] HUMAN CYTOMEGALOVIRUS-IE1 TRANSACTIVATES THE ALPHA-PROMOTER-ENHANCER VIA AN 18-BASE-PAIR REPEAT ELEMENT [J].

CHERRINGTON, JM ;

MOCARSKI, ES .

JOURNAL OF VIROLOGY, 1989, 63 (03) :1435-1440

[2] Dynamic histone H3 acetylation and methylation at human cytomegalovirus promoters during replication in fibroblasts [J].

Cuevas-Bennett, Christian ;

Shenk, Thomas .

JOURNAL OF VIROLOGY, 2008, 82 (19) :9525-9536

[3] Activation of the NF-κB pathway in human cytomegalovirus-infected cells is necessary for efficient transactivation of the major immediate-early promoter [J].

DeMeritt, IB ;

Milford, LE ;

Yurochko, AD .

JOURNAL OF VIROLOGY, 2004, 78 (09) :4498-4507

[4]

Garcia H, 2011, ONCOTARGET, V2, P783

[5] Curaxins: Anticancer Compounds that Simultaneously Suppress NF-κB and Activate p53 by Targeting FACT [J].

Gasparian, Alexander V. ;

Burkhart, Catherine A. ;

Purmal, Andrei A. ;

Brodsky, Leonid ;

Pal, Mahadeb ;

Saranadasa, Madhi ;

Bosykh, Dmitry A. ;

Commane, Mairead ;

Guryanova, Olga A. ;

Pal, Srabani ;

Safina, Alfiya ;

Sviridov, Sergey ;

Koman, Igor E. ;

Veith, Jean ;

Komar, Anton A. ;

Gudkov, Andrei V. ;

Gurova, Katerina V. .

SCIENCE TRANSLATIONAL MEDICINE, 2011, 3 (95)

[6] Human cytomegalovirus gene expression during infection of primary hematopoietic progenitor cells: A model for latency [J].

Goodrum, FD ;

Jordan, CT ;

High, K ;

Shenk, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (25) :16255-16260

[7] Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation [J].

Huang, Jing-Yi ;

Chen, Wei-Hao ;

Chang, Ya-Ling ;

Wang, Hsiao-Ting ;

Chuang, Wan-ting ;

Lee, Sheng-Chung .

NUCLEIC ACIDS RESEARCH, 2006, 34 (08) :2398-2407

[8] Detection of endogenous human cytomegalovirus in CD34(+) bone marrow progenitors [J].

Mendelson, M ;

Monard, S ;

Sissons, P ;

Sinclair, J .

JOURNAL OF GENERAL VIROLOGY, 1996, 77 :3099-3102

[9]

Mocarski E.S., 2007, FIELDSVIROLOGY, V5th, P2702

[10] Control of cytomegalovirus lytic gene expression by histone acetylation [J].

Murphy, JC ;

Fischle, W ;

Verdin, E ;

Sinclair, JH .

EMBO JOURNAL, 2002, 21 (05) :1112-1120

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