Spectrophotometric parameters of conformational states of hemoglobin and its complexes with ligands in the sturgeons

被引：5
|
作者：
Vasil'ev, AS [1 ]
Luk'yanenko, VI [1 ]
机构：
[1] Russian Acad Sci, Inst Biol Inner Waters, Borok, Yaroslavl Raion, Russia
来源：
JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY | 2000年 / 36卷 / 01期
关键词：
Evolutionary Biochemistry; Conformational State; Prosthetic Group; Great Sturgeon; Russian Sturgeon;
D O I：
10.1007/BF02890658
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Parameters of carbo-, carboxy-, oxy-, deoxy-, and methemoglobin of the human and four species of sturgeons (sterlet, Russian sturgeon, starred sturgeon, great sturgeon) were analyzed spectrophotometrically. It has been shown that in the absorption spectra of all forms of hemoglobins there is only one Soret gamma(1) band due to the prosthetic group of the pigment; the wavelength of this band is undoubtedly associated with the hemoglobin conformation. This permits the use of the band as an indicator parameter to control an initial state of the fish hemoglobin solutions, the analysis of conformational states of the pigment macromolecules, and the study of hemoglobin derivatives (its complexes with ligands).
引用
收藏
页码：5 / 10
页数：6
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