Characterisation of Verticillium dahliae by inter-simple sequence repeats identified a virulent subpopulation affecting Australian cotton

被引:2
|
作者
Dadd-Daigle, Pearl [1 ,2 ]
Collins, Damian [1 ]
Kirkby, Karen [3 ]
Roser, Sharlene [3 ]
Lonergan, Peter [3 ]
Chowdhury, Piklu Roy [2 ]
Labbate, Maurizio [2 ]
Chapman, Toni A. [1 ]
机构
[1] Elizabeth Macarthur Agr Inst, NSW Dept Primary Ind, Biosecur & Food Safety, Woodbridge Rd, Menangle, NSW 2568, Australia
[2] Univ Technol Sydney, Sch Life Sci, Harris St, Ultimo, NSW 2007, Australia
[3] NSW Dept Primary Ind, Biosecur & Food Safety, Kamilaroi Highway, Sydney, NSW 2390, Australia
关键词
Verticillium; Cotton; Gossypium hirsutum; V; dahliae; INFECTED OLIVE PLANTS; GENETIC DIVERSITY; PATHOGENICITY; POPULATIONS; WILT;
D O I
10.1007/s13313-022-00865-w
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Verticillium dahliae is an agricultural phytopathogen of notable importance around the world and is increasingly impacting the billion-dollar Australian cotton industry. In diseased Australian cotton, the VCG classification system of V. dahliae does not adequately reflect the disease symptoms observed. The non-defoliating VCG2A isolates have caused significant damage, a problem not readily observed overseas, and the defoliating VCG1A isolates have not always caused widespread severe symptoms as expected. This study examined a selection of V. dahliae isolates derived from diseased cotton plants in NSW, Australia, America and Israel and classified them into groups using Inter-Simple Sequence Repeats (ISSR). A subset of these isolates were tested in a pathogenicity assay using cotton plants. The combined results confirmed that Australia has VCG2A isolates capable of causing significant disease ("defoliating-like") and that when analysed using ISSR, they are distinct from the non-virulent VCG2A populations. Additionally, there is a group of VCG1A isolates that are able to cause significant damage to cotton plants. The ISSR analysis provides a better understanding of the V. dahliae populations circulating within Australia and is a useful tool for diagnostic use, with a potential use for diagnostic confirmation of a causative strain.
引用
收藏
页码:409 / 418
页数:10
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