Mode of action of the retrogene product SNAI1P, a SNAIL homolog, in human breast cancer cells

被引：7

作者：

Mittal, Mukul K. ^{[1
]}

Myers, Jeremy N. ^{[1
]}

Bailey, Charvann K. ^{[1
]}

Misra, Smita ^{[1
]}

Chaudhuri, Gautam ^{[1
]}

机构：

[1] Meharry Med Coll, Dept Microbial Pathogenesis & Immune Response, Nashville, TN 37208 USA

来源：

MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 03期

关键词：

SNAI1P; SNAIL; Claudin; 7; E2-box; Transcriptional repression; CtBP1; HDAC1; TRANSCRIPTIONAL REPRESSOR SNAIL; TUMOR RECURRENCE; EXPRESSION; PROTEIN; GENE; SLUG; CARCINOMA; INVASION; PROGRESSION; PROMOTER;

D O I：

10.1007/s11033-009-9492-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SNAI1P, a protein coded by a retrogene, is a member of the SNAI family of E2-box binding transcriptional repressors. To evaluate whether the mode of action of SNAI1P is similar to those of the other predominant members of the SNAI family, we studied its action on human claudin 7 (CLDN7) gene promoter which has seven E2-boxes. We over-expressed FLAG-tagged SNAI1P in MCF7 and MDA-MB-468 cells. SNAI1P inhibited the expression of CLDN7 in these recombinant cells. SNAI1P also inhibited cloned CLDN7 gene promoter activity in human breast cancer cells. ChIP assays revealed that SNAI1P is recruited on the CLDN7 gene promoter along with the co-repressor CtBP1 and the effector HDAC1. Treatment of the cells with trichostatin A, an inhibitor of HDAC1, abrogated the repressor activity of SNAI1P. These data suggest that SNAI1P inhibits CLDN7 gene promoter epigenetically in breast cancer cells through chromatin remodeling.

引用

页码：1221 / 1227

页数：7

共 31 条

[1] The Ajuba LIM domain protein is a corepressor for SNAG domain-mediated repression and participates in nucleocytoplasmic shuttling [J].

Ayyanathan, Kasirajan ;

Peng, Hongzhuang ;

Hou, Zhaoyuan ;

Fredericks, William J. ;

Goyal, Rakesh K. ;

Langer, Ellen M. ;

Longmore, Gregory D. ;

Rauscher, Frank J., III .

CANCER RESEARCH, 2007, 67 (19) :9097-9106

[2] Human SLUG does not directly bind to CtBP1 [J].

Bailey, Charvann K. ;

Misra, Smita ;

Mittal, Mukul K. ;

Chaudhuri, Gautam .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 353 (03) :661-664

[3] The Snail genes as inducers of cell movement and survival: implications in development and cancer [J].

Barrallo-Gimeno, A ;

Nieto, MA .

DEVELOPMENT, 2005, 132 (14) :3151-3161

[4] Calmodulin-like protein increases filopodia-dependent cell motility via up-regulation of myosin-10 [J].

Bennett, Richard D. ;

Mauer, Amy S. ;

Strehler, Emanuel E. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (05) :3205-3212

[5] Transmembrane proteins of tight junctions [J].

Chiba, Hideki ;

Osanai, Makoto ;

Murata, Masaki ;

Kojima, Takashi ;

Sawada, Norimasa .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2008, 1778 (03) :588-600

[6] Snail and slug play distinct roles during breast carcinoma progression [J].

Come, Christophe ;

Magnino, Fabrice ;

Bibeau, Frederic ;

Barbara, Pascal De Santa ;

Becker, Karl Friedrich ;

Theillet, Charles ;

Savagner, Pierre .

CLINICAL CANCER RESEARCH, 2006, 12 (18) :5395-5402

[7] Of snail, mice, and women [J].

Davidson, NE ;

Sukumar, S .

CANCER CELL, 2005, 8 (03) :173-174

[8] Snail in the frame of malignant tumor recurrence [J].

De Craene, Bram ;

Berx, Geert .

BREAST CANCER RESEARCH, 2006, 8 (04)

[9] The transcription factor snail mediates epithelial to mesenchymal transitions by repression of estrogen receptor-α [J].

Dhasarathy, Archana ;

Kajita, Masahiro ;

Wade, Paul A. .

MOLECULAR ENDOCRINOLOGY, 2007, 21 (12) :2907-2918

[10] The LIM protein AJUBA recruits protein arginine methyltransferase 5 to mediate SNAIL-dependent transcriptional repression [J].

Hou, Zhaoyuan ;

Peng, Hongzhuang ;

Ayyanathan, Kasirajan ;

Yan, Kai-Ping ;

Langer, Ellen M. ;

Longmore, Gregory D. ;

Rauscher, Frank J., III .

MOLECULAR AND CELLULAR BIOLOGY, 2008, 28 (10) :3198-3207

← 1 2 3 4 →