Evaluation of the efficacy and safety of the Rift Valley Fever Clone 13 vaccine in sheep

被引:103
作者
Dungu, Baptiste [1 ]
Louw, Ian [1 ]
Lubisi, Alison [2 ]
Hunter, Pamela [1 ]
von Teichman, Beate F. [1 ]
Bouloy, Michele [3 ]
机构
[1] Onderstepoort Biol Prod Ltd, ZA-0110 Onderstepoort, South Africa
[2] ARC Onderstepoort Vet Inst, ZA-0110 Onderstepoort, South Africa
[3] Inst Pasteur, Unite Genet Mol Bunyavirus, F-75724 Paris, France
关键词
Rift Valley Fever; Vaccine; Sheep; VIRUS; CATTLE; LAMBS; IMMUNOGENICITY; INFECTION; DISEASE; SEGMENT; EWES; LIVE;
D O I
10.1016/j.vaccine.2010.04.085
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The efficacy and safety of the naturally attenuated Rift Valley Fever (RVF) Clone 13 vaccine were evaluated in ovines in three different experiments involving 38 ewes at different stages of pregnancy, their offsprings and four rams. In Experiment 1, 4 rams and a total of 13 pregnant ewes were vaccinated and monitored during vaccination and after a challenge with a virulent RVF virus. The ewes were vaccinated at either 50 or 100 days of pregnancy and some were challenged after lambing. In Experiment 2, nine oestrus-synchronized ewes were vaccinated at 50 days of pregnancy and challenged at 100 days of pregnancy together with 5 unvaccinated ewes at the same stage of pregnancy. In Experiment 3, 16 oestrus-synchronized ewes were vaccinated with 3 different doses of the RVF Clone 13 vaccine and challenged together with unvaccinated pregnant ewes at either 30 or 50 days of pregnancy. The results from the three experiments indicated that the vaccine did not induce clinical manifestation of RVF such as abortion in pregnant ewes, teratogeny in their offsprings, or pyrexia in all vaccinated animals. Vaccination with RVF Clone 13 vaccine also prevented clinical RVF following virulent challenge at different stages of pregnancy while unvaccinated control ewes showed pyrexia, aborted or died of RVF. A vaccine dose-response effect was also observed. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4581 / 4587
页数:7
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