Engineering Cupriavidus necator DSM 545 for the one-step conversion of starchy waste into polyhydroxyalkanoates

被引：44

作者：

Brojanigo, Silvia ^{[1
]}

Gronchi, Nicoletta ^{[1
]}

Cazzorla, Tiziano ^{[1
]}

Wong, Tuck Seng ^{[2
,3
]}

Basaglia, Marina ^{[1
]}

Favaro, Lorenzo ^{[1
]}

Casella, Sergio ^{[1
]}

机构：

[1] Univ Padua, Dept Agron Food Nat Resources Anim & Environm DAF, Agripolis, Viale Univ 16, I-35020 Legnaro, PD, Italy

[2] Univ Sheffield, Dept Chem & Biol Engn, Sir Robert Hadfield Bldg,Mappin St, Sheffield S1 3JD, S Yorkshire, England

[3] Natl Ctr Genet Engn & Biotechnol, 113 Thailand Sci Pk,Phahonyothin Rd, Khlong Luang 12120, Pathum Mani, Thailand

来源：

BIORESOURCE TECHNOLOGY | 2022年 / 347卷

关键词：

Consolidated BioProcessing; Recombinant amylase expression; Broken rice; Sweet potato waste; 3HB; PHA; PERFORMANCE; WATER; ACID;

D O I：

10.1016/j.biortech.2021.126383

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Starch-rich by-products could be efficiently exploited for polyhydroxyalkanoates (PHAs) production. Unfortunately, Cupriavidus necator DSM 545, one of the most efficient PHAs producers, is not able to grow on starch. In this study, a recombinant amylolytic strain of C. necator DSM 545 was developed for the one-step PHAs production from starchy residues, such as broken rice and purple sweet potato waste. The glucodextranase G1d from Arthrobacter globiformis I42 and the alpha-amylase amyZ from Zunongwangia profunda SM-A87 were co-expressed into C. necator DSM 545. The recombinant C. necator DSM 545 #11, selected for its promising hydrolytic activity, produced high biomass levels with noteworthy PHAs titers: 5.78 and 3.65 g/L from broken rice and purple sweet potato waste, respectively. This is the first report on the engineering of C. necator DSM 545 for efficient amylase production and paves the way to the one-step conversion of starchy waste into PHAs.

引用

页数：7

共 49 条

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