Production and composition of extracellular polysaccharide synthesized by a Rhizobium isolate of Vigna mungo (L.) Hepper

被引：20

作者：

Mandal, Santi Mohan

Ray, Birnalendu

Dey, Satyahari

Pati, Bikas Ranjan ^{[1
]}

机构：

[1] Vidyasagar Univ, Dept Microbiol, Midnapore 721102, WB, India

[2] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India

[3] Univ Burdwan, Dept Chem, Nat Prod Lab, Burdwan 713104, W Bengal, India

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 08期

关键词：

cultural requirements; extracellular polysaccharide; growth; Rhizobium sp;

D O I：

10.1007/s10529-007-9388-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An extracellular polysaccharide (EPS) was produced by a Rhizobium sp. isolated from the root nodules of Vigna mungo (L.) Hepper. Maximum EPS production (346 mg 1(-1)) was when the yeast extract basal medium was supplemented with mannitol (1%), biotin (1.5 mg 1(-1)) and asparagine (0.3%). Ribose (53%) and mannose (47%) were the principle monomers of the EPS. Chemical, chromatographic and spectroscopic analysis showed that this polymer, which has Man(4)Rib(1) as an oligomeric subunit, has an apparent molecular mass of 750 kDa.

引用

页码：1271 / 1275

页数：5

共 16 条

[1]

BIERMANN CJ, 1988, HYDROLYSIS OTHER CLE, P27

[2] A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS [J].