Microbiome in Blood Samples From the General Population Recruited in the MARK-AGE Project: A Pilot Study

被引：29

作者：

D'Aquila, Patrizia ^{[1
]}

Giacconi, Robertina ^{[2
]}

Malavolta, Marco ^{[2
]}

Piacenza, Francesco ^{[2
]}

Buerkle, Alexander ^{[3
]}

Villanueva, Maria Moreno ^{[3
,4
]}

Dolle, Martijn E. T. ^{[5
]}

Jansen, Eugene ^{[5
]}

Grune, Tilman ^{[6
,7
]}

Gonos, Efstathios S. ^{[8
]}

Franceschi, Claudio ^{[9
,10
]}

Capri, Miriam ^{[9
,11
]}

Grubeck-Loebenstein, Beatrix ^{[12
]}

Sikora, Ewa ^{[13
]}

Toussaint, Olivier ^{[14
]}

Debacq-Chainiaux, Florence ^{[14
]}

Hervonen, Antti ^{[15
]}

Hurme, Mikko ^{[15
]}

Slagboom, P. Eline ^{[16
]}

Schoen, Christiane ^{[17
]}

Bernhardt, Juergen ^{[17
]}

Breusing, Nicolle ^{[18
]}

Passarino, Giuseppe ^{[1
]}

Provinciali, Mauro ^{[2
]}

Bellizzi, Dina ^{[1
]}

机构：

[1] Univ Calabria, Dept Biol Ecol & Earth Sci DIBEST, Arcavacata Di Rende, Italy

[2] IRCCS Sci Inst Res Hospitalizat & Healthcare, INRCA Natl Inst Hlth & Sci Ageing, Adv Technol Ctr Aging Res, Ancona, Italy

[3] Univ Konstanz, Mol Toxicol Grp, Constance, Germany

[4] Univ Konstanz, Human Performance Res Ctr, Dept Sport Sci, Constance, Germany

[5] Natl Inst Publ Hlth & Environm, Ctr Hlth Protect, Bilthoven, Netherlands

[6] German Inst Human Nutr Potsdam Rehbruecke DIfE, Dept Mol Toxicol, Nuthetal, Germany

[7] NutriAct Competence Cluster Nutr Res Berlin Potsd, Nuthetal, Germany

[8] Natl Hellen Res Fdn, Inst Biol Med Chem & Biotechnol, Athens, Greece

[9] Univ Bologna, Alma Mater Studiorum, Dept Expt Diagnost & Specialty Med, Bologna, Italy

[10] Lobachevsky Univ, Inst Informat Technol Math & Mech, Nizhnii Novgorod, Russia

[11] Univ Bologna, Alma Mater Res Inst Global Challenges & Climate C, Interdept Ctr, Bologna, Italy

[12] Univ Innsbruck, Res Inst Biomed Aging Res, Innsbruck, Austria

[13] Polish Acad Sci, Nencki Inst Expt Biol, Lab Mol Bases Ageing, Warsaw, Poland

[14] Univ Namur, Namur Res Inst Life Sci Narilis, Res Unit Cellular Biol URBC, Namur, Belgium

[15] Univ Tampere, Med Sch, Tampere, Finland

[16] Leiden Univ, Med Ctr, Dept Mol Epidemiol, Leiden, Netherlands

[17] BioTeSys GmbH, Schelztorstr, Esslingen, Germany

[18] Univ Hohenheim, Inst Nutr Med, Dept Appl Nutr Sci Dietet, Stuttgart, Germany

来源：

FRONTIERS IN MICROBIOLOGY | 2021年 / 12卷

关键词：

blood microbiome; 16S rRNA gene; geographic origin; aging; free fatty acids; leukocytes; insulin; glucose; HUMAN GUT MICROBIOME; BACTERIA; PERMEABILITY; BIOMARKERS; DYSBIOSIS; ACIDS;

D O I：

10.3389/fmicb.2021.707515

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The presence of circulating microbiome in blood has been reported in both physiological and pathological conditions, although its origins, identities and function remain to be elucidated. This study aimed to investigate the presence of blood microbiome by quantitative real-time PCRs targeting the 16S rRNA gene. To our knowledge, this is the first study in which the circulating microbiome has been analyzed in such a large sample of individuals since the study was carried out on 1285 Randomly recruited Age-Stratified Individuals from the General population (RASIG). The samples came from several different European countries recruited within the EU Project MARK-AGE in which a series of clinical biochemical parameters were determined. The results obtained reveal an association between microbial DNA copy number and geographic origin. By contrast, no gender and age-related difference emerged, thus demonstrating the role of the environment in influencing the above levels independent of age and gender at least until the age of 75. In addition, a significant positive association was found with Free Fatty Acids (FFA) levels, leukocyte count, insulin, and glucose levels. Since these factors play an essential role in both health and disease conditions, their association with the extent of the blood microbiome leads us to consider the blood microbiome as a potential biomarker of human health.

引用

页数：9

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