Paper-based upconversion fluorescence resonance energy transfer biosensor for sensitive detection of multiple cancer biomarkers

被引：49
作者：
Xu, Sai [1 ,2 ]
Dong, Biao [1 ]
Zhou, Donglei [1 ]
Yin, Ze [1 ]
Cui, Shaobo [1 ]
Xu, Wen [1 ]
Chen, Baojiu [2 ]
Song, Hongwei [1 ]
机构：
[1] Jilin Univ, Coll Elect Sci & Engn, State Key Lab Integrated Optoelect, Changchun 130012, Peoples R China
[2] Dalian Maritime Univ, Dept Phys, Dalian 116026, Peoples R China
来源：
SCIENTIFIC REPORTS | 2016年 / 6卷
基金：
中国国家自然科学基金;
关键词：
CARCINOEMBRYONIC ANTIGEN; NANOPARTICLES; NANOCRYSTALS; PLATFORM; DEVICE; SENSOR; NANOPHOSPHORS; IMMUNOASSAY; ENHANCEMENT; PROGNOSIS;
D O I：
10.1038/srep23406
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A paper-based upconversion fluorescence resonance energy transfer assay device is proposed for sensitive detection of CEA. The device is fabricated on a normal filter paper with simple nanoprinting method. Upconversion nanoparticles tagged with specific antibodies are printed to the test zones on the test paper, followed by the introduction of assay antigen. Upconversion fluorescence measurements are directly conducted on the test zones after the antigen-to-antibody reactions. Furthermore, a multi-channel test paper for simultaneous detection of multiple cancer biomarkers was established by the same method and obtained positive results. The device showed high anti-interfere, stability, reproducible and low detection limit (0.89 ng/mL), moreover it is very easy to fabricate and operate, which is a promising prospect for a clinical point-of-care test.
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页数：9
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