Interaction between PII and NifA in Azospirillum brasilense Sp7

被引：8
|
作者：
Chen, SF [1 ]
Du, JP
Wu, LX
Zhao, YS
Li, JL
机构：
[1] China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R China
[2] China Agr Univ, Natl Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2003年 / 48卷 / 02期
关键词：
Azospirillum brasilense; yeast two-hybrid system; P-II; NifA;
D O I：
10.1360/03tb9035
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The interaction between P-II and NifA in A. brasilense Sp7 was investigated by using the yeast two-hybrid system. Our experimental results showed that P-II directly interacted with the entire NifA protein and its N-terminal domain, but did not interact with the central domain and the C-terminal domain of NifA. No interaction happened if glnB coding for P-II was frame-shift mutated. Pz, a homolog of P-II, had no interation with NifA.
引用
收藏
页码：170 / 174
页数：5
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